Exercise by lifelong voluntary wheel running reduces subsarcolemmal and interfibrillar mitochondrial hydrogen peroxide production in the heart
Exercise by lifelong voluntary wheel running reduces subsarcolemmal and interfibrillar mitochondrial hydrogen peroxide production in the heart
复制标题
DOI：
10.1152/ajpregu.00396.2005
复制
发表时间：
2005-12-01
影响因子：
2.8
通讯作者：
Leeuwenburgh, C
中科院分区：
文献类型：
--
作者：
Judge, S;Jang, YM;Leeuwenburgh, C
有证据表明，线粒体功能障碍和氧化剂的产生，与氧化损伤的积累，有助于衰老过程。定期的身体活动可以延迟发病，增加平均寿命，并降低发展几种病理状态的风险。还没有研究检查与终身运动相结合的肌膜下（SSM）和原纤维间（FM）线粒体中氧化剂产生和氧化应激的年龄相关变化。因此，我们研究了Fischer 344大鼠长期自愿跑轮子是否改变了心脏SSM和IFM中过氧化氢（H2 O2）的产生、抗氧化防御和氧化损伤。在10 - 11周龄时，将大鼠随机分配到两组之一：久坐和8%食物限制（久坐; n = 20）或轮跑和8%食物限制（跑步者; n = 20）;在24月龄时处死大鼠。6个月后，跑步活动保持在平均1,145 +/- 248米/天。通过双标记水技术测定的每日能量消耗表明，跑步者平均每天消耗的能量比久坐的大鼠多70%。长期自愿轮运行显着减少H2 O2生产从SSM（-10.0%）和IFM（-9.6%），并增加每日能量消耗（千焦/天）显着的运动员相比，久坐不动的控制。此外，MnSOD活性显着降低SSM和IFM从轮亚军，这可能反映了线粒体超氧化物产生的减少。其他主要的抗氧化酶（谷胱甘肽过氧化物酶和过氧化氢酶）和谷胱甘肽水平的活动没有改变车轮运行。尽管线粒体氧化剂产生减少，但在两组之间没有检测到氧化应激水平（4-羟基-2-壬烯醛修饰的蛋白质、蛋白质羰基和丙二醛）的显著差异。长期运动的健康益处可能至少部分是由于线粒体氧化剂产生的减少;然而，我们无法检测到氧化应激的几个选定参数的显着减少。
Evidence suggests that mitochondrial dysfunction and oxidant production, in association with an accumulation of oxidative damage, contribute to the aging process. Regular physical activity can delay the onset of morbidity, increase mean lifespan, and reduce the risk of developing several pathological states. No studies have examined age- related changes in oxidant production and oxidative stress in both subsarcolemmal (SSM) and interfibrillar ( FM) mitochondria in combination with lifelong exercise. Therefore, we investigated whether long- term voluntary wheel running in Fischer 344 rats altered hydrogen peroxide (H2O2) production, antioxidant defenses, and oxidative damage in cardiac SSM and IFM. At 10 - 11 wk of age, rats were randomly assigned to one of two groups: sedentary and 8% food restriction (sedentary; n = 20) or wheel running and 8% food restriction (runners; n = 20); rats were killed at 24 mo of age. After the age of 6 mo, running activity was maintained at an average of 1,145 +/- 248 m/ day. Daily energy expenditure determined by doubly labeled water technique showed that runners expended on average similar to 70% more energy per day than the sedentary rats. Long-term voluntary wheel running significantly reduced H2O2 production from both SSM (- 10.0%) and IFM (- 9.6%) and increased daily energy expenditure (kJ/day) significantly in runners compared with sedentary controls. Additionally, MnSOD activity was significantly lowered in SSM and IFM from wheel runners, which may reflect a reduction in mitochondrial superoxide production. Activities of the other major antioxidant enzymes (glutathione peroxidase and catalase) and glutathione levels were not altered by wheel running. Despite the reduction in mitochondrial oxidant production, no significant differences in oxidative stress levels (4-hydroxy-2-nonenal- modified proteins, protein carbonyls, and malondialdehyde) were detected between the two groups. The health benefits of chronic exercise may be, at least partially, due to a reduction in mitochondrial oxidant production; however, we could not detect a significant reduction in several selected parameters of oxidative stress.