The TCA Pathway is an Important Player in the Regulatory Network Governing Vibrio alginolyticus Adhesion Under Adversity.
The TCA Pathway is an Important Player in the Regulatory Network Governing Vibrio alginolyticus Adhesion Under Adversity.
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TCA 途径是调控逆境下溶藻弧菌粘附的监管网络中的重要角色
DOI：
10.3389/fmicb.2016.00040
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发表时间：
2016
影响因子：
5.2
通讯作者：
Zheng J
中科院分区：
文献类型：
--
作者：
Huang L;Huang L;Yan Q;Qin Y;Ma Y;Lin M;Xu X;Zheng J
黏附是溶藻弧菌感染初期的关键步骤；因此，了解溶藻弧菌粘附的潜在机制并确定环境因素是否有影响是很重要的。更好地了解这一过程可能有助于制定减少感染的预防措施。在我们之前的研究中，我们提供了第一个RNA-seq数据，这些数据来自于在胁迫条件下培养的藻毒弧菌，导致粘连降低。基于RNA-seq数据，我们发现三羧酸循环（Tricarboxylic acid cycle, TCA pathway）可能与粘附密切相关。环境与TCA途径的相互作用可能会改变粘附。为了验证这一点，我们进行了生物信息学分析、定量Real-Time PCR （qPCR）、RNAi和体外粘附试验，同时对溶藻弧菌进行了不同的胁迫处理，包括温度、pH、盐度和饥饿。qPCR证实了TCA通路相关基因的表达，增强了测序数据的可靠性。这些基因的沉默能够降低溶藻弧菌的粘附能力。溶藻弧菌的粘附性受环境因素的影响很大，TCA途径对某些环境胁迫，特别是pH和饥饿的变化很敏感。结果表明：(1)TCA途径在溶藻弧菌粘附过程中起关键作用；(2)TCA途径对环境胁迫敏感。
Adhesion is a critical step in the initial stage of Vibrio alginolyticus infection; therefore, it is important to understand the underlying mechanisms governing the adhesion of V. alginolyticus and determine if environmental factors have any effect. A greater understanding of this process may assist in developing preventive measures for reducing infection. In our previous research, we presented the first RNA-seq data from V. alginolyticus cultured under stress conditions that resulted in reduced adhesion. Based on the RNA-seq data, we found that the Tricarboxylic acid cycle (TCA pathway) might be closely related to adhesion. Environmental interactions with the TCA pathway might alter adhesion. To validate this, bioinformatics analysis, quantitative Real-Time PCR (qPCR), RNAi, and in vitro adhesion assays were performed, while V. alginolyticus was treated with various stresses including temperature, pH, salinity, and starvation. The expression of genes involved in the TCA pathway was confirmed by qPCR, which reinforced the reliability of the sequencing data. Silencing of these genes was capable of reducing the adhesion ability of V. alginolyticus. Adhesion of V. alginolyticus is influenced substantially by environmental factors and the TCA pathway is sensitive to some environmental stresses, especially changes in pH and starvation. Our results indicated that (1) the TCA pathway plays a key role in V. alginolyticus adhesion: (2) the TCA pathway is sensitive to environmental stresses.