THE EVOLUTIONARILY CONSERVED KRUPPEL-ASSOCIATED BOX DOMAIN DEFINES A SUBFAMILY OF EUKARYOTIC MULTIFINGERED PROTEINS
THE EVOLUTIONARILY CONSERVED KRUPPEL-ASSOCIATED BOX DOMAIN DEFINES A SUBFAMILY OF EUKARYOTIC MULTIFINGERED PROTEINS
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DOI：
10.1073/pnas.88.9.3608
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发表时间：
1991-05-01
影响因子：
11.1
通讯作者：
MARTIAL, JA
中科院分区：
文献类型：
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作者：
BELLEFROID, EJ;PONCELET, DA;MARTIAL, JA
我们之前已经证明，人类基因组包括数百个基因，编码与Kruppel锌指蛋白相关的假定因子，Kruppel锌指蛋白调节果蝇的分割。我们在此报道，这些基因中约有三分之一编码蛋白质，这些蛋白质在其N末端的非指状部分有一个非常保守的区域，大约有75个氨基酸。在许多先前描述的手指蛋白中都发现了同源区域，包括小鼠ZFP-1和非洲爪哇Xfin。我们将这个区域命名为与Kruppel相关的盒子(KRAB)。这个结构域有可能形成两个两栖动物的阿尔法螺旋。对“动物园”斑点的Southern杂交分析表明，与Kruppel相关的盒子在进化过程中高度保守。Northern印迹分析表明，这些基因在大多数成人组织中都有表达，并且在人髓系细胞体外终末分化过程中表达下调。
We have previously shown that the human genome includes hundreds of genes coding for putative factors related to the Kruppel zinc-finger protein, which regulates Drosophila segmentation. We report herein that about one-third of these genes code for proteins that share a very conserved region of about 75 amino acids in their N-terminal nonfinger portion. Homologous regions are found in a number of previously described finger proteins, including mouse Zfp-1 and Xenopus Xfin. We named this region the Kruppel-associated box (KRAB). This domain has the potential to form two amphiphathic alpha-helices. Southern blot analysis of "zoo" blots suggests that the Kruppel-associated box is highly conserved during evolution. Northern blot analysis shows that these genes are expressed in most adult tissues and are down-regulated during in vitro terminal differentiation of human myeloid cells.