Physical characterization and evaluation of HPLC columns packed with superficially porous particles
Physical characterization and evaluation of HPLC columns packed with superficially porous particles
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DOI：
10.1002/jssc.201000251
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发表时间：
2010-09-01
影响因子：
3.1
通讯作者：
Colon, Luis A.
中科院分区：
文献类型：
--
作者：
Baker, Jared S.;Vinci, John C.;Colon, Luis A.
用表面多孔填充材料（KinetexTM 1.7和2.6 μ m C18颗粒）填充的两个HPLC柱在它们的物理性质和性能特征方面进行评价。将这些柱与填充有低于2 μ m的完全多孔材料的柱和填充有2.7 μ mC18表面多孔填料的HaloTM柱进行比较。填充有表面多孔颗粒的柱显示出比完全多孔的亚2 μ m颗粒的分布更窄的尺寸分布。根据表面积、孔径和比孔体积评价KinetexTM颗粒的物理特性。对四种不同色谱柱的总孔隙率、外部孔隙率、内部孔隙率和壳孔隙率进行了评价和比较。Kinetex（TM）柱的比渗透率显示接近Kozeny卡曼方程预测的值。评价了所有四种色谱柱的色谱性能，并使用Knox方程进行了比较。填充有2.6和2.7 μ m表面多孔材料的柱显示低于2的降低的板高度，而低于2 μ m的颗粒显示2.2和以上的值。
A Two H PLC columns packed with superficially porous packing material (Kinetex (TM) 1.7 and 2.6 mu m C18 particles) were evaluated in terms of their physical properties and performance characteristics. These columns were compared to a column packed with a sub-2 mu m totally porous material and to a Halo (TM) column packed with 2.7 mu m C18 superficially porous packing. The columns packed with superficially porous particles displayed a comparably narrower size distribution, which is narrower than the distribution of the totally porous sub-2 mu m particles. Physical characteristics of the Kinetex (TM) particles were evaluated in terms of surface area, pore diameter, and specific pore volume. Total, external, internal, and shell porosities among the four different columns were evaluated and compared. The specific permeability for the Kinetex (TM) columns showed values close to those predicted by the Kozeny Carman equation. All four columns were evaluated in terms of their chromatographic performance and compared using the Knox equation. The columns packed with the 2.6 and 2.7 mu m superficially porous materials showed reduced plate heights below 2, whereas the sub-2 mu m particles showed values of 2.2 and above.