How few cancer cells can be detected by positron emission tomography? A frequent question addressed by an in vitro study
How few cancer cells can be detected by positron emission tomography? A frequent question addressed by an in vitro study
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DOI：
10.1007/s00259-005-0038-6
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发表时间：
2006-06-01
影响因子：
9.1
通讯作者：
Kristjansen, PEG
中科院分区：
文献类型：
--
作者：
Fischer, BM;Olsen, MWB;Kristjansen, PEG
目的：正电子发射断层扫描（PET）在癌症诊断和治疗中得到了广泛的应用，但PET检测肿瘤需要多少恶性细胞？研究方法：将浓度为10（4）至10（7）的三种人癌细胞系[胶质母细胞瘤和两种亚型的小细胞肺癌（SCLC）]接种在六孔板或塑料管上，并在体外用[F-18]氟脱氧葡萄糖（FDG）处理。在PET/CT扫描仪和校准的孔计数器中测量FDG保留。临床情况是模拟使用一个圆柱体模与背景浓度的FDG.Results：被发现的理论检测限约为10（5）恶性细胞。在圆柱体模中，检测限增加了10倍。胶质母细胞瘤细胞系的FDG保留显著高于SCLC细胞系的活性。通过PET和γ计数器测量的FDG保留与细胞的数量密切相关，并且发现了线性关系。讨论：PET的检测极限在10（5）至10（6）个恶性细胞的量级。实验设置是强大的，非常适合作为一个平台，进一步研究影响PET的检测限的因素。
Purpose: Positron emission tomography ( PET) has gained widespread use in cancer diagnosis and treatment, but how many malignant cells are required for a tumour to be detected by PET?Methods: Three human cancer cell lines [ glioblastoma and two subtypes of small cell lung cancer (SCLC)] in concentrations from 10(4) to 10(7) were seeded on six-well plates or plastic tubes and treated with [F-18] fluorodeoxyglucose (FDG) in vitro. FDG retention was measured in a PET/CT scanner and in a calibrated well counter. The clinical situation was simulated using a cylinder phantom with a background concentration of FDG.Results: The theoretical detection limit was found to be around 10(5) malignant cells. In a cylinder phantom the detection limit was increased by a factor of 10. The FDG retention by the glioblastoma cell line was significantly higher than the activity of the SCLC cell line. FDG retention measured by PET and a gamma counter was closely correlated to the number of cells and a linear relationship was found.Discussion: The detection limit of PET is in the magnitude of 10(5) to 10(6) malignant cells. The experimental set-up was robust and well suited as a platform for further investigations of factors influencing the detection limit of PET.