S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization
S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization
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DOI：
10.1590/s1806-83242010000200002
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发表时间：
2010-06-01
影响因子：
2.5
通讯作者：
Cury, Jaime Aparecido
中科院分区：
文献类型：
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作者：
Ccahuana-Vásquez, Renzo Alberto;Cury, Jaime Aparecido
本研究的目的是验证S.变形菌生物膜的形成模拟了在口腔中发生的暴露于蔗糖的“盛宴-饥荒”事件，显示了对抗微生物剂的剂量-反应敏感性，并允许评价具有防龋潜力的物质。S.在37 ℃、10%CO2下，在牛牙釉质板上生长变形菌UA 159生物膜5天。为了验证该模型，每天用0.012、0.024和0.12%（具有公认的抗牙斑效果的浓度）的二葡糖酸氯己定（CHX）和0.05%NaF（具有公认的抗龋齿效果的浓度）处理生物膜2次。CHX能显著降低釉质脱矿率、细菌存活率和生物量（p < 0.05）。0.05% NaF无抗菌作用，但与0.12% CHX对釉质脱矿有相似的抑制作用（p < 0.05）。开发的模型有可能评估生物膜生长和釉质脱矿物质的影响。
The aim of this study was to validate a model of S. mutans biofilm formation, which simulated 'feast-famine' episodes of exposure to sucrose that occur in the oral cavity, showed dose-response susceptibility to antimicrobials and allowed the evaluation of substances with anticaries potential. S. mutans UA159 biofilms were grown for 5 days on bovine enamel slabs at 37 degrees C, 10% CO2. To validate the model, the biofilms were treated 2x/day with chlorhexidine digluconate (CHX) at 0.012, 0.024 and 0.12% (concentration with recognized anti-plaque effect) and 0.05% NaF (concentration with recognized anti-caries effect). CHX showed dose-response effect decreasing biomass, bacterial viability and enamel demineralization (p < 0.05). Whereas, 0.05% NaF did not show antimicrobial effect but had similar effect to that of 0.12% CHX decreasing enamel demineralization (p < 0.05). The model developed has potential to evaluate the effect of substances on biofilm growth and on enamel demineralization.