Subcellular localization of SARS-CoV structural proteins.
Subcellular localization of SARS-CoV structural proteins.
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DOI：
10.1007/978-0-387-33012-9_51
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发表时间：
2006
影响因子：
--
通讯作者：
Hogue BG
中科院分区：
文献类型：
--
作者：
Lopez LA;Jones A;Arndt WD;Hogue BG
冠状病毒是一种有包膜的病毒，在感染细胞的内质网-高尔基体中间室（ERGIC）的细胞内膜上组装。1 S和M是病毒包膜的主要成分。E蛋白是包膜的次要组分，但在病毒组装中起重要作用。2冠状病毒包膜的形成不依赖于核衣壳。仅E和M蛋白的表达足以形成许多冠状病毒的病毒样颗粒（VLP）。3-5 N蛋白是一种多功能的磷蛋白，它使病毒基因组发生变性，并在病毒装配中发挥作用。6，7 N似乎也参与病毒RNA复制和/或转录。8，9本研究的目的是提供一个全面的亚细胞定位的主要SARS-CoV结构蛋白。在BHK-21细胞中表达S、M、E和N基因，并通过间接免疫荧光显微镜分析蛋白质的定位。共分析的蛋白质与特定的细胞器标记的内质网（ER）和高尔基体。此外，M和E蛋白与ERGIC的细胞标志物共定位。N蛋白仍在胞质内。的S，M和E蛋白被发现集中到高尔基体区域，虽然一些S似乎也被运送到细胞表面。
Coronaviruses are enveloped viruses that assemble at intracellular membranes of the endoplasmic reticulum–Golgi intermediate compartment (ERGIC) in infected cells. 1 S and M are the main components of the viral envelope. The E protein is a minor component of the envelope, but plays an important role in virus assembly. 2 Coronavirus envelope formation is nucleocapsid independent. Expression of only the E and M proteins is sufficient for the formation of virus-like-particles (VLPs) of many coronaviruses. 3-5 The N protein is a multifunctional phosphoprotein that encapsidates the viral genome and plays a role in virus assembly. 6, 7 N also appears to be involved in viral RNA replication and/or transcription. 8, 9The aim in this study was to provide a comprehensive view of the subcellular localization of the main SARS-CoV structural proteins. The S, M, E, and N genes were expressed in BHK-21 cells, and localization of the proteins was analyzed by indirect immunofluorescence microscopy. The proteins were co-analyzed with specific organelle markers for the endoplasmic reticulum (ER) and Golgi. Additionally, the M and E proteins were co-localized with a cellular marker for the ERGIC. The N protein remained cytoplasmic. The S, M, and E proteins were found to concentrate to the Golgi region, although some S appeared to also be transported to the cell surface.