Ultrafast laser-scanning optical resolution photoacoustic microscopy at up to 2 million A-lines per second
Ultrafast laser-scanning optical resolution photoacoustic microscopy at up to 2 million A-lines per second
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DOI：
10.1117/1.jbo.23.12.126502
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发表时间：
2018-12-01
影响因子：
3.5
通讯作者：
Beard, Paul C.
中科院分区：
文献类型：
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作者：
Allen, Thomas John;Spurrell, Josh;Beard, Paul C.
使用脉冲激励的光学分辨率光声显微镜（OR-PAM）的成像速度从根本上受到距离模糊条件的限制，该条件定义了最大激光脉冲重复频率（PRF）。为了在这个理论上限下工作并最大限度地提高采集速度，定制的光纤激光器能够在高达2 MHz的PRF下工作，并结合基于固定式光纤超声传感器的快速激光扫描光学OR-PAM系统。当获得1600万A线并在2 MHz的PRF下操作激光器时，在8 s内对小鼠耳的大面积（10 mm × 10 mm）进行了成像。与之前报道的最快的OR-PAM系统相比，这相当于成像速度提高了四倍。在高成像帧率下操作的能力也允许捕获血液动力学事件，如血流。人们认为，该系统为利用OR-PAM进行高通量成像和可视化动态生理事件提供了机会。(c)提交人。由SPIE在知识共享署名3.0未移植许可下发布。
The imaging speed of optical resolution photoacoustic microscopy (OR-PAM) using pulsed excitation is fundamentally limited by the range ambiguity condition, which defines the maximum laser pulse repetition frequency (PRF). To operate at this theoretical upper limit and maximize acquisition speed, a custom-built fiber laser capable of operating at a PRF of up to 2 MHz was combined with a fast laser scanning optical OR-PAM system based on a stationary fiber-optic ultrasound sensor. A large area (10 mm x 10 mm) of the mouse ear was imaged within 8 s, when acquiring 16 million A-lines and operating the laser at a PRF of 2 MHz. This corresponds to a factor of four improvement in imaging speed compared to the fastest OR-PAM system previously reported. The ability to operate at high-imaging frame rates also allows the capture of hemodynamic events such as blood flow. It is considered that this system offers opportunities for high throughput imaging and visualizing dynamic physiological events using OR-PAM. (c) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.