Dynamic characteristics of AHLs-secreting strain Aeromonas sp. A-L2 and its bioaugmentation during quinoline biodegradation
Dynamic characteristics of AHLs-secreting strain Aeromonas sp. A-L2 and its bioaugmentation during quinoline biodegradation
复制标题
AHLs 分泌菌株气单胞菌的动态特性。
DOI：
10.1111/jam.14530
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发表时间：
2020
影响因子：
4
通讯作者：
Zhang A. N.
中科院分区：
文献类型：
--
作者：
Gao M.;Liu Y. J.;Liu Z.;Li H. T.;Zhang A. N.
目的探讨基于微生物生物强化和群体感应（quorum sensing，QS）效应的环境友好型污染物处理方法。对从活性污泥系统中分离到的A-L2（A-L2）进行了讨论。液相色谱-质谱联用结果表明，菌株A-L2分泌的AHLs主要为N-丁酰-高丝氨酸内酯（C4-HSL）和N-己酰-高丝氨酸内酯（C6-HSL），菌株Ochrobactrumsp. LC-1（LC-1）由这些化合物诱导。菌株LC-1的胞外多聚物分泌主要受C6-HSL的影响，生物膜形成能力主要受C6-HSL或C4-HSL（60 μg l-1）的影响。对菌株A-L2分泌AHLs的最适条件进行了研究。在菌株LC-1降解喹啉的过程中，得到分泌AHLs的菌株A-L2的支持，降解时间大大缩短。AHLs分泌菌株A-L2可以作为生物强化和污染物降解过程中AHLs的持续供应者。研究意义和影响A-L2菌株基于QS效应对喹啉生物降解的生物强化过程，将为AHLs的生物强化和污染物的生物降解提供一定的理论和实践意义。大规模应用
AimsIn order to probe a more environmentally friendly method of pollutant treatment based on microbial bioaugmentation and quorum sensing (QS) effects.Methods and ResultsThe dynamic characteristics and QS effects of the acylated homoserine lactones (AHLs)‐secreting strainAeromonassp. A‐L2 (A‐L2), which was isolated from the activated sludge system, was discussed. According to the liquid chromatography‐mass spectrometry results, N‐butyryl‐homoserine lactone (C4‐HSL) and N‐hexanoyl‐homoserine lactone (C6‐HSL) were the major AHLs secreted by strain A‐L2, and the swarming of strainOchrobactrumsp. LC‐1 (LC‐1) was induced by these compounds. The extracellular polymeric substance secretion of the strain LC‐1 was mainly led by C6‐HSL, and the biofilm formation ability was mainly influenced by C6‐HSL or C4‐HSL (60 μg l−1). The optimal AHLs secretion conditions of strain A‐L2 were also studied. Drawing support from the AHLs‐secreting strain A‐L2 during quinoline degradation by strain LC‐1, the degradation time was greatly shortened.ConclusionsHence, AHLs‐secreting strain A‐L2 can be useful as an AHLs continuous supplier during bioaugmentation and pollutant biodegradation.Significance and Impact of the StudyThe bioaugmentation process of strain A‐L2 on quinoline biodegradation based on QS effects would lay a certain theoretical and practical significance for large‐scale applications.