Suppressive effects of milk-derived lactoferrin on psychological stress in adult rats
Suppressive effects of milk-derived lactoferrin on psychological stress in adult rats
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DOI：
10.1016/j.brainres.2004.09.015
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发表时间：
2004-12-10
期刊：
影响因子：
2.9
通讯作者：
Harada, E
中科院分区：
文献类型：
--
作者：
Kamemori, N;Takeuchi, T;Harada, E
乳铁蛋白（LF）被称为铁结合糖蛋白。研究表明，牛 LF (bLF) 可通过血液转运至脑脊液，但其对中枢神经系统 (CNS) 的生理作用尚不清楚。在这项研究中，研究了 bLF 对成年大鼠心理困扰的抑制作用。腹腔注射 bLF (100 mg/kg) 可减少条件恐惧诱发的冰冻试验和高架十字迷宫试验中的应激行为。有趣的是，电足电击（FS）预处理增强了 bLF 的抑制作用。 bLF 在高架十字迷宫测试中的这种抑制作用可以通过用纳洛酮（一种阿片受体拮抗剂）以 1 mg/kg（腹腔注射）剂量进行预处理来逆转。 N-omega-硝基-L-精氨酸甲酯 (L-NAME) 是一种一氧化氮合酶 (NOS) 抑制剂，也能阻断 bLF 和足部休克的抑制作用。此外，联合应用低剂量bLF（30 mg/kg，腹膜内）和L-精氨酸（30和100 mg/kg，腹膜内）对心理压力显示出显着的增强作用。这些结果表明 bLF 对心理困扰具有抑制作用，尤其是在中等压力的情况下。此外，表明 bLF 可能通过一氧化氮合酶激活来激活内源性阿片能系统。 (C) 2004 Elsevier B.V. 保留所有权利。
Lactoferrin (LF) is known as an iron-binding glycoprotein. It has been shown that bovine LF (bLF) is transported into cerebrospinal fluid via blood although its physiological effects in the central nervous system (CNS) are still unclear. In this study, a suppressive effect of bLF on psychological distress was investigated in adult rats. Intraperitoneal injection of bLF (100 mg/kg) reduced stressful behaviors in a conditioned fear-induced freezing test and an elevated plus-maze test. Interestingly, the suppressive effect of bLF was enhanced by pretreatment with electric foot-shock (FS). This suppressive effect of bLF in the elevated plus-maze test was reversed by pretreatment with naloxone, an opioid receptor antagonist, at a dose of 1 mg/kg (ip). N-omega-nitro-L-arginine methyl ester (L-NAME), a nitric oxide synthase (NOS) inhibitor, also blocked the suppressive effect of bLF and foot-shock. In addition, combined application of a low dose of bLF (30 mg/kg, ip) and L-arginine (30 and 100 mg/kg, ip) showed significant potentiated effects on psychological stress. These results suggest that bLF has suppressive effects on psychological distress, especially under the condition of moderate stress. Furthermore, it is suggested that bLF possibly activates an endogenous opioidergic system via nitric oxide synthase activation. (C) 2004 Elsevier B.V. All rights reserved.