SCANNING ELECTRON-MICROSCOPY OF EPIPLEXUS MACROPHAGES (KOLMER CELLS) IN DOG
SCANNING ELECTRON-MICROSCOPY OF EPIPLEXUS MACROPHAGES (KOLMER CELLS) IN DOG
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DOI：
10.1002/cne.901610205
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发表时间：
1975-01-01
影响因子：
2.5
通讯作者：
ALLEN, DJ
中科院分区：
文献类型：
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作者：
ALLEN, DJ
通过标准技术用缓冲醛灌注幼犬（6-14周）。从第四脑室完整取出下髓帆和脉络组织，进行后渗、脱水、临界点干燥，涂上钯金，并在剑桥Stereoscan S4扫描电子显微镜中进行检查。本研究描述了大量表现出极端多态性的上丛（Kolmer细胞）巨噬细胞。由于这些细胞及其突起的多形性，基于外部形态的分类是不切实际的。TEM显示其内部结构为巨噬细胞。某些室管膜细胞表面似乎完全或部分缺乏纤毛，出现水泡样结构和球状突起。
Young dogs (6–14 weeks) were perfused with buffered aldehydes by standard techniques. The inferior medullary velum and tela choroidea were removed intact from the fourth ventricle, post‐osmicated, dehydrated, critical point dried, coated with palladium‐gold and examined in a Cambridge Stereoscan S4 scanning electron microscope. Selected samples after thorough scanning were routinely prepared for transmission electron microscopy (TEM).This study depicts numerous epiplexus (Kolmer cell) macrophages that exhibit extreme polymorphism. Because of the pleomorphic nature of these cells and their processes, classification based on external morphology is not practical. TEM shows their internal structure to be that of a macrophage. Bleb‐like structures and bulbous projections appear to emerge from certain ependymal cell surfaces which seem to be completely or partially devoid of cilia.