COMPARISON OF SENSITIVITY OF BHK-21 AND MURINE NEUROBLASTOMA-CELLS IN THE ISOLATION OF A STREET STRAIN RABIES VIRUS
COMPARISON OF SENSITIVITY OF BHK-21 AND MURINE NEUROBLASTOMA-CELLS IN THE ISOLATION OF A STREET STRAIN RABIES VIRUS
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DOI：
10.1128/jcm.25.8.1456-1458.1987
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发表时间：
1987-08-01
影响因子：
9.4
通讯作者：
TRIMARCHI, CV
中科院分区：
文献类型：
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作者：
RUDD, RJ;TRIMARCHI, CV
比较了 BHK-21 (C-13) 和鼠神经母细胞瘤 (C-1300；克隆 NA) 细胞分离少量狂犬病街头株的敏感性。在出现狂犬病临床症状之前处死的小鼠的大脑悬浮液用于刺激弱阳性诊断样本。将细胞培养分离的结果与断奶小鼠中直接荧光抗体测试和病毒分离的结果进行比较。神经母细胞瘤细胞比 BHK-21 细胞对街头狂犬病病毒更敏感。神经母细胞瘤细胞病毒分离、小鼠接种试验和荧光抗体试验均显示出相当的敏感性。
The sensitivities of BHK-21 (C-13) and murine neuroblastoma (C-1300; clone NA) cells for the isolation of small quantities of a street strain rabies virus were compared. Suspension of brain from mice sacrificed prior to the onset of clinical signs of rabies were used to stimulate weakly positive diagnostic specimens. The results of cell culture isolation were compared with those of the direct fluorescent-antibody test and virus isolation in weanling mice. Neuroblastoma cells were more sensitive to the street strain rabies virus than were BHK-21 cells. Neuroblastoma cell virus isolation, the mouse inoculation test, and the fluorescent-antibody test all showed comparable sensitivity.