A Bioinspired Ultraporous Nanofiber-Hydrogel Mimic of the Cartilage Extracellular Matrix
A Bioinspired Ultraporous Nanofiber-Hydrogel Mimic of the Cartilage Extracellular Matrix
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DOI：
10.1002/adhm.201600867
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发表时间：
2016-12-21
影响因子：
10
通讯作者：
Zenobi-Wong, Marcy
中科院分区：
文献类型：
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作者：
Formica, Florian A.;Ozturk, Ece;Zenobi-Wong, Marcy
软骨细胞外基质（ECM）的真正仿生可以极大地有助于我们在机械要求高，经常发炎的环境中再生这种组织的能力。关节软骨是由细胞和纤维蛋白包埋在亲水性聚合物网络中的复合组织。这里，使用聚阴离子官能化藻酸盐来模拟天然ECM的糖胺聚糖组分。为了产生纤维状组分，使用聚（ε-己内酯）在-78 ° C心轴上的低温电纺丝，随后通过O-2等离子体处理，以产生稳定的、超多孔的和无定形的网状物。在这项研究中，可以通过将软骨细胞/藻酸盐溶液渗透到纤维网中，然后进行物理交联，来制造厚度超过1.5 mm的载有细胞的纤维增强复合材料支架。原纤维组分显著增强软骨诱导性，但机械强度较弱的硫酸化藻酸盐水凝胶。这允许软骨细胞产生富含糖胺聚糖和II型胶原的基质以及复合材料在体内的存活。为了进一步增强该系统，电纺组件负载有地塞米松，从而保护细胞免受IL-1 β介导的炎症损伤。
A true biomimetic of the cartilage extracellular matrix (ECM) could greatly contribute to our ability to regenerate this tissue in a mechanically demanding, often inflamed environment. Articular cartilage is a composite tissue made of cells and fibrillar proteins embedded in a hydrophilic polymeric meshwork. Here, a polyanionic functionalized alginate is used to mimic the glycosaminoglycan component of the native ECM. To create the fibrillar component, cryoelectrospinning of poly(epsilon-caprolactone) on a - 78 degrees C mandrel, subsequently treated by O-2 plasma, is used to create a stable, ultraporous and hydrophillic nanofiber network. In this study, cell-laden, fiber-reinforced composite scaffolds thicker than 1.5 mm can be created by infiltrating a chondrocyte/alginate solution into the fiber mesh, which is then physically cross-linked. The fibrillar component significantly reinforces the chondroinductive, but mechanically weak sulfated alginate hydrogels. This allows the production of a glycosaminoglycan- and collagen type II-rich matrix by the chondrocytes as well as survival of the composite in vivo. To further enhance the system, the electrospun component is loaded with dexamethasone, which protected the cells from an IL-1 beta-mediated inflammatory insult.