The antioxidant capacity of erythrocyte concentrates is increased during the first week of storage and correlated with the uric acid level
The antioxidant capacity of erythrocyte concentrates is increased during the first week of storage and correlated with the uric acid level
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DOI：
10.1111/vox.12563
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发表时间：
2017-10-01
期刊：
影响因子：
2.7
通讯作者：
Prudent, M.
中科院分区：
文献类型：
--
作者：
Bardyn, M.;Maye, S.;Prudent, M.
背景和目的红细胞在冷藏过程中会受到损伤，这部分取决于它们通过激活抗氧化防御来平衡氧化应激的能力。本研究的目的是监测抗氧化能力（AOP）在红细胞浓缩物（ECs）在冷藏过程中，在生理盐水腺嘌呤葡萄糖甘露醇（SAGM）的添加剂溶液中制备6个ECs，并在43天。AOP电化学定量使用一次性电极条，并与从比色测定获得的结果进行比较。还记录了血液学数据、溶血数据和细胞外尿酸浓度。此外，一个动力学模型被开发，以提取定量动力学数据的AOP behaviors.ResultsThe AOP的总EC和他们的细胞外样品达到最大值后，1周的存储前腐烂，并达到一个平台，如所示的电化学测量。用比色测定法证实了观察到的趋势。尿酸对细胞外AOP有主要贡献。有趣的是，AOP和尿酸水平与性别的donors.ConclusionThe在存储的第一周期间AOP的显着增加表明，红细胞的影响早期修改他们的环境。AOP行为反映了细胞外尿酸水平调整后代谢活性的变化。了解红细胞的来源、供体间的变异性以及AOP对红细胞的影响可能有利于红细胞的储存质量，这将需要进一步研究。
Background and ObjectivesRed blood cells (RBCs) suffer from lesions during cold storage, depending in part on their ability to counterbalance oxidative stress by activating their antioxidant defence. The aim of this study was to monitor the antioxidant power (AOP) in erythrocyte concentrates (ECs) during cold storage.Materials and MethodsSix ECs were prepared in saline-adenine-glucose-mannitol (SAGM) additive solution and followed during 43days. The AOP was quantified electrochemically using disposable electrode strips and compared with results obtained from a colorimetric assay. Haematological data, data on haemolysis and the extracellular concentration of uric acid were also recorded. Additionally, a kinetic model was developed to extract quantitative kinetic data on the AOP behaviour.ResultsThe AOP of total ECs and their extracellular samples attained a maximum after 1week of storage prior to decaying and reaching a plateau, as shown by the electrochemical measurements. The observed trend was confirmed with a colorimetric assay. Uric acid had a major contribution to the extracellular AOP. Interestingly, the AOP and uric acid levels were linked to the sex of the donors.ConclusionThe marked increase in AOP during the first week of storage suggests that RBCs are impacted early by the modification of their environment. The AOP behaviour reflects the changes in metabolism activity following the adjustment of the extracellular uric acid level. Knowing the origin, interdonor variability and the effects of the AOP on the RBCs could be beneficial for the storage quality, which will have to be further studied.