The Effect of Laser and Ultrasound Synchronization in Photo-Mediated Ultrasound Therapy.
The Effect of Laser and Ultrasound Synchronization in Photo-Mediated Ultrasound Therapy.
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DOI：
10.1109/tbme.2020.2985648
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Wang X
中科院分区：
文献类型：
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作者：
Qin Y;Yu Y;Xie X;Zhang W;Fu J;Paulus YM;Yang X;Wang X
光介导超声治疗（PUT）是一种新型、无创、无药物、高选择性和精确的抗血管技术。PUT通过应用同步的纳秒激光脉冲和超声脉冲，精确地促进靶微血管中的空化活动来去除微血管。激光和超声波之间的同步对于PUT的结果至关重要。通过理论模拟和实验研究，评价了脉冲激光与超声脉冲之间的同步性对超声空化过程中空化活动的影响。通过使用一个理论模型，我们发现，当激光脉冲和超声脉冲同步，使所产生的光声波覆盖的超声波的稀疏相位时，空化活动增强。然后通过体外研究验证了这一发现，其中通过使用被动空化检测器监测空化。此外，我们证明了在兔体内的PUT治疗结果与激光和超声之间的同步直接相关。只有当激光和超声在体内适当同步时，才能观察到抗血管效应。当激光诱导的光声波与超声波的稀疏相位重叠时，PUT更有效。本研究系统地研究了PUT的同步化效应，对于进一步了解PUT的作用机理，提高PUT的处理效率具有重要意义。
Photo-mediated ultrasound therapy (PUT) is a novel, non-invasive, agent-free, highly selective, and precise anti-vascular technique. PUT removes microvessels through promoting cavitation activity precisely in targeted microvessels by applying synchronized nanosecond laser pulses and ultrasound bursts. The synchronization between laser and ultrasound is critical to the outcome of PUT. Through theoretical simulation and experimental study, the effect of synchronization between laser pulses and ultrasound bursts on cavitation activity during PUT is evaluated. By using a theoretical model, we found that cavitation activity was enhanced when laser pulses and ultrasound bursts were synchronized such that the produced photoacoustic wave overlaid the rarefactional phase of the ultrasound wave. This finding was then verified through in vitro studies where cavitation was monitored by using a passive cavitation detector. Furthermore, we demonstrated that the in vivo treatment outcome of PUT in rabbits was directly related to the synchronization between laser and ultrasound. The anti-vascular effect could only be observed when laser and ultrasound were properly synchronized in vivo. PUT is more efficient when the laser-induced photoacoustic wave overlays the rarefactional phase of the ultrasonic wave. This is a systematic study to investigate the synchronization effect of PUT, which would be significant for further understanding the mechanism and further improving the treatment efficiency of PUT.