Single-Cell Analysis of Growth in Budding Yeast and Bacteria Reveals a Common Size Regulation Strategy
Single-Cell Analysis of Growth in Budding Yeast and Bacteria Reveals a Common Size Regulation Strategy
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DOI：
10.1016/j.cub.2015.11.067
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发表时间：
2016-02-08
期刊：
影响因子：
9.2
通讯作者：
Amir, Ariel
中科院分区：
文献类型：
--
作者：
Soifer, Ilya;Robert, Lydia;Amir, Ariel
为了保持恒定的细胞大小，分裂细胞必须协调细胞周期事件与细胞生长。这种协调长期以来一直被认为依赖于确定细胞周期进展的大小阈值的存在[1]。在芽殖酵母中，大小控制在G1/S转变[2]。与这一假设一致，出生时的大小影响G1期的时间：较小的细胞具有较长的G1期[3]。尽管如此，即使细胞出生时较小，G1期也较长，但补偿是不完美的，它们仍然在较小的细胞尺寸下发芽。在细菌中，最近的几项研究表明，大小控制的增量模型，其中通过添加恒定体积来控制大小（与大小阈值相反），能够定量解释四种不同细菌物种的实验数据[4-7]。在这里，我们报告的实验结果的芽殖酵母酿酒酵母，发现，令人惊讶的是，在这种生物体中的细胞大小控制是非常好地描述的增量模型，这表明一个共同的战略，细胞大小控制与细菌。此外，我们认为，对于S。酿酒厂的“体积增量”不是从出生到分裂，而是在两个萌芽事件之间。
To maintain a constant cell size, dividing cells have to coordinate cell-cycle events with cell growth. This coordination has long been supposed to rely on the existence of size thresholds determining cell-cycle progression [1]. In budding yeast, size is controlled at the G1/S transition [2]. In agreement with this hypothesis, the size at birth influences the time spent in G1: smaller cells have a longer G1 period [3]. Nevertheless, even though cells born smaller have a longer G1, the compensation is imperfect and they still bud at smaller cell sizes. In bacteria, several recent studies have shown that the incremental model of size control, in which size is controlled by addition of a constant volume (in contrast to a size threshold), is able to quantitatively explain the experimental data on four different bacterial species [4-7]. Here, we report on experimental results for the budding yeast Saccharomyces cerevisiae, finding, surprisingly, that cell size control in this organism is very well described by the incremental model, suggesting a common strategy for cell size control with bacteria. Additionally, we argue that for S. cerevisiae the "volume increment'' is not added from birth to division, but rather between two budding events.