Two forms of the basic fibroblast growth factor receptor-like mRNA are expressed in the developing mouse brain.
Two forms of the basic fibroblast growth factor receptor-like mRNA are expressed in the developing mouse brain.
复制标题
发育中的小鼠大脑中表达两种形式的碱性成纤维细胞生长因子受体样 mRNA。
DOI：
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发表时间：
1990
影响因子：
11.1
通讯作者：
O. Bernard
中科院分区：
文献类型：
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作者：
Hugh H. Reid;A. Wilks;O. Bernard
胚胎神经上皮细胞在成熟动物中产生中枢神经系统的组成部分。为了研究小鼠中枢神经系统的早期发育，我们试图从10天大小鼠胚胎神经管的神经上皮中分离生长因子受体。由于许多生长因子受体的蛋白酪氨酸激酶家族的成员，我们已经使用聚合酶链反应扩增mRNA序列从10天大的小鼠胚胎神经上皮细胞，这些序列之间的核苷酸序列的两个高度保守的氨基酸基序从蛋白酪氨酸激酶的催化域。通过使用这种技术，我们已经分离出一个克隆编码的小鼠碱性成纤维细胞生长因子受体（bFGF-R），以及较短的形式，这种mRNA。后者的cDNA包括75%的bFGF-R的cDNA克隆从永生化的神经上皮细胞系分离。这种变体mRNA，在此指定为N-bFGF-R，似乎在发育早期的神经元细胞中以较高水平表达。bFGF-R是一个多基因家族的成员，如Southern印迹分析和该家族另外两个成员的克隆所证明的。
The embryonic neuroepithelium gives rise to the components of the central nervous system in the mature animal. To study the early development of the murine central nervous system we have sought to isolate growth factor receptors from the neuroepithelium of the neural tube of 10-day-old mouse embryos. Because many growth factor receptors are members of the protein-tyrosine kinase family, we have used the polymerase chain reaction to amplify mRNA sequences from 10-day-old mouse embryo neuroepithelium; these sequences lie between the nucleotide sequences of two highly conserved amino acid motifs from the catalytic domain of protein-tyrosine kinases. By using this technique we have isolated a clone encoding the murine basic fibroblast growth factor receptor (bFGF-R), as well as a shorter form of this mRNA. This latter cDNA comprised 75% of the bFGF-R cDNA clones isolated from the immortalized neuroepithelial cell lines. This variant mRNA, designated here as N-bFGF-R, appears to be expressed at higher levels in neuronal cells in early stages of development. The bFGF-R is a member of a multigene family, as demonstrated by Southern blot analysis and the cloning of two other members of this family.