MORPHOLOGICAL HETEROGENEITY AMONG SALMONELLA LIPOPOLYSACCHARIDE CHEMOTYPES IN SILVER-STAINED POLYACRYLAMIDE GELS
MORPHOLOGICAL HETEROGENEITY AMONG SALMONELLA LIPOPOLYSACCHARIDE CHEMOTYPES IN SILVER-STAINED POLYACRYLAMIDE GELS
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DOI：
10.1128/jb.154.1.269-277.1983
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发表时间：
1983-01-01
影响因子：
3.2
通讯作者：
BROWN, TM
中科院分区：
文献类型：
--
作者：
HITCHCOCK, PJ;BROWN, TM
银染聚丙烯酰胺凝胶电泳分析了不同脂多糖（LPS）化学型的saltinase突变体之间LPS的形态异质性。LPS化学型的生化差异反映在纯化LPS的独特谱中。全细胞裂解物中的LPS谱对于每种化学型也是独特的。（通过优先Ag染色LPS的方法和通过全细胞裂解物的蛋白酶K消化来评估全细胞裂解物。蛋白酶K消化的裂解物的Ag染色的LPS谱与同源纯化的LPS相似，并且可用于在纯化前初步表征LPS的化学型。LPS组成的生化变化可以在Ag染色的聚丙烯酰胺凝胶中检测到。
The morphological heterogeneity of lipopolysaccharides (LPS) among salmonella mutants with different LPS chemotypes was analyzed in Ag-stained polyacrylamide gels. The biochemical differences in the LPS chemotypes were reflected in the unique profiles of the purified LPS. The LPS profiles in the whole-cell lysates were also unique for each chemotype. (Whole-cell lysates were assessed by a method which preferentially Ag stains LPS and by a proteinase K digest of whole-cell lysates. The Ag-stained LPS profiles of proteinase K-digested lysates were similar to the homologous purified LPS and could be used to preliminarily characterize the LPS chemotype before purification. Biochemical variation in LPS composition can be detected in Ag-stained polyacrylamide gels.