Shoot regeneration of Campanula carpatica Jacq. (Campanulaceae) via callus phase
Shoot regeneration of Campanula carpatica Jacq. (Campanulaceae) via callus phase
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DOI：
10.1016/s0304-4238(01)00312-0
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发表时间：
2002-02-28
影响因子：
4.3
通讯作者：
Serek, M
中科院分区：
文献类型：
--
作者：
Frello, S;Stummann, BM;Serek, M
风铃草的成年植株。从通过来自两个品种：白色统一和蓝色统一的子叶的愈伤组织再生的芽获得。对白色均匀和蓝色均匀的愈伤组织和不定芽的产生进行了优化，采用了一种培养基，即含有2，4-D（2，4-dichlorophenoxyacetic acid）、BAP（6-benzylaminopurine）、NAA（alpha-naphthalene acetic acid）。芽转移到无菌培养基诱导根的生长。当根已经发育时，将小植株转移到土壤中，并在温室条件下发育成成熟植株。外植体成熟率最高为2.8%。这两个品种之间的整体再生效率，即外植体形成生根芽的数量没有显着差异。第三个品种，蓝剪辑，产生了一些芽，其中没有形成根。从下胚轴或幼叶再生植物的尝试没有成功。(C)2002 Elsevier Science B. V.保留所有权利。
Adult plants of Campanula carpatica Jacq. were obtained from shoots regenerated via callus derived from cotyledons of two cultivars: White Uniform and Blue Uniform. An optimized procedure used one medium, containing 2,4-D (2,4-dichlorophenoxyacetic acid), BAP (6-benzylaminopurine), NAA (alpha-naphthalene acetic acid) for producing both callus and shoots from White Uniform and Blue Uniform. Transfer of shoots to hormone-free medium induced the growth of roots. When roots had developed, the plantlets were transferred to soil, and developed into mature plants under greenhouse conditions. The highest achieved rate of explants resulting in mature plants was 2.8%. There were no significant differences in overall regeneration efficiency, i.e. number of explants forming rooted shoots between these two cultivars. A third cultivar, Blue Clips, produced a few shoots of which none formed roots. Attempts to regenerate plants from hypocotyls or young leaves were not successful. (C) 2002 Elsevier Science B.V. All rights reserved.