Defining the T cell antigen proteome of wasp venom
Defining the T cell antigen proteome of wasp venom
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DOI：
10.1111/j.1365-2222.2006.02569.x
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发表时间：
2006-10-01
影响因子：
6.1
通讯作者：
Ogg, G.
中科院分区：
文献类型：
--
作者：
Aslam, A.;Kessler, B.;Ogg, G.
背景虽然T细胞功能的调节被认为是在毒液免疫治疗过程中成功获得临床耐受性的重要因素，但对黄蜂毒液特异性T细胞抗原的作用知之甚少。目的全面研究黄蜂毒液的T细胞蛋白质组特征，为今后基于T细胞的免疫治疗方法的发展提供依据。方法利用黄蜂毒液过敏个体外周血单个核细胞和IL-4 ELISPOT分析，研究了T细胞对全毒液和凝胶过滤/离子交换分离毒液的反应。活性组分的纯化和鉴定使用高灵敏度的电喷雾离子阱质谱法。结果黄蜂毒液过敏个体在体外可检测到完整的黄蜂毒液特异性T细胞，并表现出快速的IL-4效应功能。T细胞对凝胶过滤/离子交换分离毒液的反应主要是对磷脂酶A(1)、透明质酸酶和抗原5的反应。结论虽然胡蜂毒液中可能存在多种T细胞抗原，但主要反应是与已知的ige结合蛋白一致的蛋白。
Background While modulation of T cell function is believed to be important in the successful acquisition of clinical tolerance during venom immunotherapy, little is known of the role of wasp venom specific T cell antigens.Objective We sought comprehensively to characterize the T cell proteome for wasp venom to facilitate the future development of T cell-based immunotherapeutic approaches.Methods Using peripheral blood mononuclear cells from wasp venom-allergic individuals and IL-4 ELISPOT analysis, we characterized T cell responses to whole venom and gel filtration/ion exchange-fractionated venom. Reactive fractions were purified and identified using highly sensitive electrospray ion-trap mass spectrometry.Results Wasp venom-allergic individuals have detectable whole wasp venom-specific T cells directly ex vivo, which show rapid IL-4 effector function. T cell responses to gel filtration/ion exchange fractionated venom were dominated by responses to phospholipase A(1), hyaluronidase and antigen 5.Conclusion Although it is likely that there are many T cell antigens within wasp venom, the main responses are to proteins coincident with the known IgE-binding proteins.