A fluorescence-based demonstration of intestinal villi and epithelial cell in chickens fed dietary silicic acid powder including bamboo vinegar compound liquid.
A fluorescence-based demonstration of intestinal villi and epithelial cell in chickens fed dietary silicic acid powder including bamboo vinegar compound liquid.
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饲喂含有竹醋复合液的日粮硅酸粉的鸡肠绒毛和上皮细胞的荧光演示。
DOI：
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发表时间：
2012
影响因子：
2
通讯作者：
K. Yamauchi
中科院分区：
文献类型：
--
作者：
J. Ruttanavut;Y. Matsumoto;K. Yamauchi
本研究探讨了复合氨基酸竹醋液对家禽生长和肠道组织学的影响。选用48只7日龄雄性萨努基Cochin鸡，在商品饲料中添加0、0.1、0.2和0.3%的SPV，自由采食112 d。体重增加倾向于随着饲料SPV浓度的增加而改善，尽管这些结果在统计学上不显著（P<0.1）。光镜观察发现，与对照组相比，0.2%SPV组空肠绒毛高度（P<0.01）、十二指肠和空肠绒毛面积（P<0.05）均显著增加。在0.2%和0.3%SPV组中，十二指肠和空肠内的细胞有丝分裂也增加。扫描电子显微镜显示，与对照组相比，0.2%和0.3% SPV组的十二指肠和空肠绒毛顶面上的突起细胞数量显著增加。0.3%SPV组鸡的增重和肠绒毛肥大的组织学改变最高。与对照组相比，十二指肠隐窝中细胞有丝分裂和突起细胞的荧光显微镜图像清楚地证实了激活蛋白2α（AP-2α）和增殖细胞核抗原（PCNA）的阳性反应。本研究结果表明，饲料SPV刺激上皮细胞的吸附，从而激活细胞增殖和自我更新，并调节细胞周期调节因子AP-2α和PCNA的表达，导致更高的体重增加。因此，我们可以得出结论，0.3%的饲料SPV浓度对于促进家禽生长是理想的。
This study investigates the combined effect of silicic acid and bamboo vinegar compound liquid (SPV) on the growth and intestinal histological alterations in poultry. Forty-eight 7-day-old male Sanuki Cochin chickens were fed a commercial mash diet supplemented with SPV at 0, 0.1, 0.2, and 0.3% level ad libitum for 112 days. Body weight gain tended to improve with increased concentrations of dietary SPV, although these results were not statistically significant (P<0.1). Tissue observation by light microscopy revealed that the jejunal villus height (P<0.01) and duodenal and jejunal villus area (P<0.05) increased in the 0.2 and 0.3% SPV groups, respectively, compared with the control. Cell mitosis within the duodenum and jejunum also increased in the 0.2 and 0.3% SPV groups. Scanning electron microscopy revealed a prominent increase in the number of protuberant cells on the villus apical surface of the duodenum and jejunum for the 0.2 and 0.3% SPV groups compared with the control. Poultry in the 0.3% SPV group had the highest body weight gain and hypertrophied histological alterations of intestinal villi. Fluorescent microscopic images of cell mitosis and protuberant cells in the duodenal crypt clearly confirmed positive reactions for the activator protein 2α (AP-2α) and proliferating cell nuclear antigen (PCNA), compared with the control. The present results indicate that dietary SPV stimulates adsorption by the epithelial cells, which activate cell proliferation and self-renewal and regulate the expression of cell cycle regulators AP-2α and PCNA, resulting in higher body weight gain. Thus, we can conclude that a concentration of 0.3% dietary SPV is ideal for promoting growth in poultry.
影响因子：
8
作者：
P. Stabach;M. Thiyagarajan;G. Woodfield;R. Weigel
通讯作者：
P. Stabach;M. Thiyagarajan;G. Woodfield;R. Weigel