Lactobacillus rhamnosus strain GG reduces aflatoxin B1 transport, metabolism, and toxicity in caco-2 cells
Lactobacillus rhamnosus strain GG reduces aflatoxin B1 transport, metabolism, and toxicity in caco-2 cells
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DOI：
10.1128/aem.02944-06
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发表时间：
2007-06-01
影响因子：
4.4
通讯作者：
Turner, P. C.
中科院分区：
文献类型：
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作者：
Gratz, S.;Wu, Q. K.;Turner, P. C.
益生菌鼠李糖乳杆菌 GG 能够结合强效肝癌物质黄曲霉毒素 B (AFB1)，从而可能限制其在肠道中的快速吸收。在这项研究中，我们研究了 GG 在适应表达细胞色素 P-450 (CYP) 3A4 的 Caco-2 细胞中体外降低 AFB 可用性的潜力，从而可以评估转运和毒性。在所有研究之前，Caco-2 细胞在跨膜过滤器上以汇合单层生长 21 天。通过高效液相色谱法测量培养基中的AFB水平。在 CYP 3A4 诱导的单层细胞中，在与 GG (1 X 10(10) 和 5 X 10(10) CFU/ml， 分别）。 1 小时后，GG（1 X 10(10) 和 5 x 10(10) CFU/ml）分别结合添加的 AFB(1) 的 40.1% +/- 8.3% 和 61.0% +/- 6.0%。 AFB 在 24、48 和 72 小时后分别使跨上皮阻力显着降低 30.1% (P = 0.01)、49.4% (P = 0.004) 和 64.4% (P < 0.001)。 24 小时后与 1 X 10(10) CFU/ml GG 共孵育可防止 AFB(1) 诱导的 24 小时 (P = 0.002) 和 48 小时 (P = 0.04) 时跨上皮电阻的降低。 DNA 断裂在仅用 AFB 处理的细胞中很明显，但在与 1 X 10(10) 或 5 X 10(10) CFU/ml GG 共孵育的细胞中则不然。 GG 减少了 Caco-2 模型中 AFB1 的摄取并防止膜和 DNA 损伤。这些数据表明 GG 对预防饮食中黄曲霉毒素暴露具有有益作用。
The probiotic Lactobacillus rhamnosus GG is able to bind the potent hepatocarcinogen aflatoxin B, (AFB1) and thus potentially restrict its rapid absorption from the intestine. In this study we investigated the potential of GG to reduce AFB, availability in vitro in Caco-2 cells adapted to express cytochrome P-450 (CYP) 3A4, such that both transport and toxicity could be assessed. Caco-2 cells were grown as confluent monolayers on transmembrane filters for 21 days prior to all studies. AFB, levels in culture medium were measured by high-performance liquid chromatography. In CYP 3A4-induced monolayers, AFB(1) transport from the apical to the basolateral chamber was reduced from 11.1% +/- 1.9% to 6.4% +/- 2.5% (P = 0.019) and to 3.3% +/- 1.8% (P = 0.002) within the first hour in monolayers coincubated with GG (1 X 10(10) and 5 X 10(10) CFU/ml, respectively). GG (1 X 10(10) and 5 x 10(10) CFU/ml) bound 40.1% +/- 8.3% and 61.0% +/- 6.0% of added AFB(1) after 1 h, respectively. AFB, caused significant reductions of 30.1% (P = 0.01), 49.4% (P = 0.004), and 64.4% (P < 0.001) in transepithelial resistance after 24, 48, and 72 h, respectively. Coincubation with 1 X 10(10) CFU/ml GG after 24 h protected against AFB(1)-induced reductions in transepithelial resistance at both 24 h (P = 0.002) and 48 h (P = 0.04). DNA fragmentation was apparent in cells treated only with AFB, cells but not in cells coincubated with either 1 X 10(10) or 5 X 10(10) CFU/ml GG. GG reduced AFB1 uptake and protected against both membrane and DNA damage in the Caco-2 model. These data are suggestive of a beneficial role of GG against dietary exposure to aflatoxin.