Paracetamol affects the expression of detoxification- and reproduction-related genes and alters the life traits of Daphnia magna
Paracetamol affects the expression of detoxification- and reproduction-related genes and alters the life traits of Daphnia magna
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扑热息痛影响解毒和繁殖相关基因的表达并改变大型溞的生命特征
DOI：
10.1007/s10646-020-02199-z
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发表时间：
2020-05-01
期刊：
影响因子：
2.7
通讯作者：
Nie, Xiangping
中科院分区：
文献类型：
--
作者：
Ding, Rui;Liu, Sijia;Nie, Xiangping
扑热息痛（APAP）是一种广泛使用的非甾体抗炎药，在水环境中检出率较高。然而，关于APAP对水生无脊椎动物的毒性效应和解毒机制的信息有限。在本研究中，大型蚤（Daphnia magna）的生活特征（例如，研究了APAP慢性暴露（0-5000 μg/L）21 d后，对中华绒螯蟹（P-gp，EcR，Vtg）的影响，以及急性暴露（96 h）对中华绒螯蟹（P-gp，Vtg）、HR 96、CYP 360 A8、CYP 314、MRP 4、EcR和MRP 4基因表达的影响。结果表明，黄粉虫的换羽次数、第一窝卵天数和第一次产卵天数均显著高于对照。在50 μg/L浓度的APAP慢性染毒试验中，大鼠脑组织的形态发生了变化。在急性实验中，HR 96的转录表达在APAP暴露24和48 h后上调。在CYP 360 A8、CYP 314、MRP 4和P-gp的表达方面也观察到类似的性能。然而，随着暴露时间延长至96 h，HR 96的诱导减少，甚至在某些情况下逆转。这可能表明水蚤的防御系统在短时间暴露后被激活，或在较长时间暴露后变得适应。APAP暴露对生殖相关基因表达也有影响，且与暴露时间和浓度有关。总的来说，
Paracetamol (APAP) is a widely used non-steroidal anti-inflammatory drug and has been frequently detected in aquatic environment. However, limited information is provided about the toxic effects and detoxification mechanism of APAP in aquatic invertebrates. In the present study, the change of life traits of Daphnia magna (e.g., body length, growth rate and reproduction) was investigated under the chronic APAP exposure (0–5000 μg/L) for 21 day, and the effects of APAP on the expression of the detoxification- and reproduction-related genes including HR96, CYP360A8, CYP314, MRP4, P-gp, EcR and Vtg in the acute exposure (up to 96 h) were also determined. Results showed that the molting frequency, days to the first brood and days to the first egg production of D. magna were affected under the 50 μg/L concentration of APAP in the chronic exposure test. In the acute test, the transcriptional expression of HR96 was up-regulated under APAP exposure for 24 and 48 h. Similar performances were also observed in the expression of CYP360A8, CYP314, MRP4 and P-gp. However, with exposure time extended to 96 h, the induction of HR96 decreased or even reversed in some cases. It may indicate that the defense system in Daphnia is activated for a short time of exposure or becomes adaptive after longer term of exposure. APAP exposure also affected reproduction-related genes expression, which was related to the exposure time and concentration of APAP. In summary,