Biological effects of cobalt-chromium nanoparticles and ions on dural fibroblasts and dural epithelial cells
Biological effects of cobalt-chromium nanoparticles and ions on dural fibroblasts and dural epithelial cells
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DOI：
10.1016/j.biomaterials.2013.01.023
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发表时间：
2013-05-01
期刊：
影响因子：
14
通讯作者：
Ingham, Eileen
中科院分区：
文献类型：
--
作者：
Behl, Bharat;Papageorgiou, Iraklis;Ingham, Eileen
金属对金属全椎间盘置换术的引入促使研究评估钴铬（CoCr）纳米颗粒对硬脑膜细胞的影响。将从硬脑膜分离的猪成纤维细胞和上皮细胞与临床相关的CoCr纳米颗粒和由颗粒产生的离子一起培养24小时，剂量高达121 μ m（3）/细胞。在4天内评估细胞活力和促炎细胞因子的产生。在24小时评价颗粒诱导细胞中氧化应激的能力。CoCr颗粒及其离子以剂量依赖性方式显著降低硬脑膜上皮细胞的活力，但不降低成纤维细胞的活力。这两种细胞类型分泌IL-8，以响应60.5 μ m（3）（上皮细胞）和121 μ m（3）（成纤维细胞，上皮细胞）/细胞剂量的颗粒暴露。在任何剂量下，在两种细胞类型中均未观察到IL-6的显著释放。暴露24小时后，在50 μ m（3）/细胞的两种细胞类型中诱导活性氧。数据表明硬脑膜上皮细胞和成纤维细胞对CoCr纳米颗粒/离子毒性的抗性存在新的差异，并证明了颗粒的炎症潜力。这些数据有助于更好地理解使用金属对金属全椎间盘假体的潜在生物学后果。(C)2013爱思唯尔有限公司保留所有权利。
The introduction of metal-on-metal total disc replacements motivated studies to evaluate the effects of cobalt-chromium (CoCr) nanoparticles on cells of the dura mater. Porcine fibroblasts and epithelial cells isolated from the dura mater were cultured with clinically-relevant CoCr nanoparticles and the ions, generated by the particles over 24 h, at doses up to 121 mu m(3)per cell. Cell viability and production of proinflammatory cytokines was assessed over 4 days. The capacity of the particles to induce oxidative stress in the cells was evaluated at 24 h. The CoCr particles and their ions significantly reduced the viability of the dural epithelial cells in a dose-dependent manner but not the fibroblasts. Both cell types secreted IL-8 in response to particle exposure at doses of 60.5 mu m(3) (epithelial cells) and 121 mu m(3) (fibroblasts, epithelial cells) per cell. No significant release of IL-6 was observed in both cell types at any dose. Reactive oxygen species were induced in both cell types at 50 mu m(3) per cell after 24 h exposure. The data suggested novel differences in the resistance of the dural epithelial cells and fibroblasts to CoCr nanoparticle/ion toxicity and demonstrated the inflammatory potential of the particles. The data contributes to a greater understanding of the potential biological consequences of the use of metal-on-metal total disc prostheses. (C) 2013 Elsevier Ltd. All rights reserved.