2 REACTIONS ARE SIMULTANEOUSLY CATALYZED BY A SINGLE ENZYME - THE ARGININE-DEPENDENT SIMULTANEOUS FORMATION OF 2 PRODUCTS, ETHYLENE AND SUCCINATE, FROM 2-OXOGLUTARATE BY AN ENZYME FROM PSEUDOMONAS-SYRINGAE
2 REACTIONS ARE SIMULTANEOUSLY CATALYZED BY A SINGLE ENZYME - THE ARGININE-DEPENDENT SIMULTANEOUS FORMATION OF 2 PRODUCTS, ETHYLENE AND SUCCINATE, FROM 2-OXOGLUTARATE BY AN ENZYME FROM PSEUDOMONAS-SYRINGAE
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DOI：
10.1016/0006-291x(92)91081-z
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发表时间：
1992-10-30
影响因子：
3.1
通讯作者：
MORINO, Y
中科院分区：
文献类型：
--
作者：
FUKUDA, H;OGAWA, T;MORINO, Y
从Pseudomonas syringaepv.phaseolilicolaPK2中分离出的单一酶同时催化两个反应，即以2:1的摩尔比从2-酮戊二酸形成乙烯和琥珀酸。在主反应中，2-酮戊二酸被双氧化产生一分子乙烯和三分子二氧化碳。在子反应中，2-酮戊二酸和L-精氨酸均被单氧化，分别产生琥珀酸加二氧化碳和L-羟基精氨酸，后者进一步转化为胍和L-δ1-吡咯啉-5-羧酸酯。我们提出了整个反应的双回路机制，其中希夫碱结构中的 L-精氨酸和 2-酮戊二酸的结合产生了两个反应的共同中间体。
A single enzyme isolated fromPseudomonas syringaepv.phaseolicolaPK2 simultaneously catalyzed two reactions, namely, the formation of ethylene and succinate from 2-oxoglutarate, at a molar ratio of 2:1. In the main reaction, 2-oxoglutarate was dioxygenated to produce one molecule of ethylene and three molecules of carbon dioxide. In the sub-reaction, both 2-oxoglutarate and L-arginine were mono-oxygenated to yield succinate plus carbon dioxide and L-hydroxyarginine, respectively, the latter being further transformed to guanidine and L-δ1-pyrroline-5-carboxylate. We propose a dual-circuit mechanism for the entire reaction, in which the binding of L-arginine and 2-oxoglutarate in a Schiff-base structure generates a common intermediate for two reactions.