The role of chaperone complex HSP90-SGT1-RAR1 as the associated machinery for hybrid inviability between Nicotiana gossei Domin and N. tabacum L.
The role of chaperone complex HSP90-SGT1-RAR1 as the associated machinery for hybrid inviability between Nicotiana gossei Domin and N. tabacum L.
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DOI：
10.1016/j.gene.2021.145443
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发表时间：
2021-02-01
期刊：
影响因子：
3.5
通讯作者：
Mino, Masanobu
中科院分区：
文献类型：
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作者：
Katsuyama, Yushi;Doi, Mizuho;Mino, Masanobu
用烟草种间杂种F-1代的两个细胞系GTH 4和GTH 4S。gossei x N. tabacum）进行比较分析，以寻找影响杂种不育性的遗传因素。两种细胞系在37 ℃下增殖，但在转移到26 ℃后，GTH 4开始死亡，类似于F-1杂交幼苗，而GTH 4S存活。由于细胞死亡需要基因和蛋白质的重新表达，我们比较了两种细胞系之间表达的蛋白质谱，发现NgSGT 1，伴侣复合物（HSP 90-SGT 1-RAR 1）的辅伴侣，在GTH 4中表达，但在GTH 4S中不表达。农杆菌介导的NgSGT 1的瞬时表达诱导了烟草叶片中的细胞死亡，但NtSGT 1没有诱导。tabacum，这表明其可能的作用，在混合无效。N.烟草也被NgRAR 1的瞬时表达诱导，但不是NtRAR 1。相比之下，三种组分的任何亲本组合的瞬时表达表明，NgRAR 1促进细胞死亡，而NtRAR 1抑制N.烟草热休克蛋白90的特异性抑制剂格尔德霉素，抑制了农杆菌渗入后GTH 4和叶组织中过敏反应样细胞死亡的进展。本研究表明，分子伴侣复合物的组分参与了烟草种间杂种的不育。
Two cultured cell lines (GTH4 and GTH4S) of a Nicotiana interspecific F-1 hybrid (N. gossei x N. tabacum) were comparatively analyzed to find genetic factors related to hybrid inviability. Both cell lines proliferated at 37 degrees C, but after shifting to 26 degrees C, GTH4 started to die similar to the F-1 hybrid seedlings, whereas GTH4S survived. As cell death requires de novo expression of genes and proteins, we compared expressed protein profiles between the two cell lines, and found that NgSGT1, a cochaperone of the chaperone complex (HSP90-SGT1-RAR1), was expressed in GTH4 but not in GTH4S. Agrobacterium-mediated transient expression of NgSGT1, but not NtSGT1, induced cell death in leaves of N. tabacum, suggesting its possible role in hybrid inviability. Cell death in N. tabacum was also induced by transient expression of NgRAR1, but not NtRAR1. In contrast, transient expression of any parental combinations of three components revealed that NgRAR1 promoted cell death, whereas NtRAR1 suppressed it in N. tabacum. A specific inhibitor of HSP90, geldanamycin, inhibited the progression of hypersensitive response-like cell death in GTH4 and leaf tissue after agroinfiltration. The present study suggested that components of the chaperone complex are involved in the inviability of Nicotiana interspecific hybrid.