Sequence analysis and comparison of avian hepatitis E viruses from Australia and Europe indicate the existence of different genotypes
Sequence analysis and comparison of avian hepatitis E viruses from Australia and Europe indicate the existence of different genotypes
复制标题
DOI：
10.1099/vir.0.007179-0
复制
发表时间：
2009-04-01
影响因子：
3.8
通讯作者：
Hess, Michael
中科院分区：
文献类型：
--
作者：
Bilic, Lvana;Jaskulska, Barbara;Hess, Michael
在澳大利亚、美国和欧洲，在患有大肝脾疾病或肝炎-脾肿大综合征的鸡中发现了禽流感病毒感染。现有数据表明它们与哺乳动物戊型肝炎病毒(HEV)有遗传关系。在本研究中，首次报道了一株澳大利亚分离株和一株欧洲分离株的近全基因组序列。此外，还确定了与其他禽类HEV的系统发育关系。对这些分离株的序列分析确定了禽类HEV之间的主要遗传差异。它们中的大多数位于开放阅读框架(ORF)1区域，但只有少数位于预测结构域的保守基序中。ORF2区的非沉默突变提示禽流感病毒分离株之间存在潜在的不同表位。最后，系统发育分析证实了它们与哺乳动物HEV的亲缘关系，并将其分为3种不同的基因类型：1(澳大利亚)、2(美国)和3(欧洲)，表明了它们的地理分布格局。
Avian hepevirus infections were detected in chickens suffering from big liver and spleen disease or hepatitis-splenomegaly syndrome in Australia, the USA and Europe. Available data indicate their genetic relationship to mammalian hepatitis E virus (HEV). In the present study, the near-complete genomic sequences of an Australian and a European isolate of avian hepatitis E virus (avian HEV) are reported for the first time. Furthermore, the phylogenetic relationship to other avian HEVs is determined. Sequence analyses of these isolates identified major genetic differences among avian HEVs. Most of them are located within the open reading frame (ORF)1 region, although only a few lie within conserved motifs of predicted domains. Non-silent mutations in the ORF2 region suggest the presence of potentially different epitopes among avian HEV isolates. Finally, phylogenetic analysis confirmed the distant relationship to mammalian HEV and additionally suggested that the avian HEVs can be separated into three different genotypes: 1 (Australia), 2 (USA) and 3 (Europe), indicating a geographical distribution pattern.