Bromodeoxyuridine amplifies the inhibitory effect of oxygen on cell proliferation.
Bromodeoxyuridine amplifies the inhibitory effect of oxygen on cell proliferation.
复制标题
溴脱氧尿苷增强氧对细胞增殖的抑制作用。
DOI：
10.1002/cyto.990090410
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Rabinovitch,PS
中科院分区：
文献类型：
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作者：
Poot,M;Schindler,D;Kubbies,M;Hoehn,H;Rabinovitch,PS
使用BrdUrd-Hoechst方法分析各种氧浓度与BrdUrd取代的DNA在细胞增殖方面的相互作用。当氧浓度高于5%时，人二倍体成纤维细胞样细胞和羊水成纤维细胞样细胞的增殖率降低，这是由于非周期细胞增加和细胞永久停滞在细胞周期的G2期。在35%的氧气的增加noncyling细胞分数和G2中的永久性逮捕是强烈依赖于浓度的BrdUrd。因此，将BrdUrd掺入DNA中会放大增加氧浓度对细胞增殖的不利影响。这种扩增的机制可能涉及对DNA的自由基攻击，类似于BrdUrd的辐射增敏作用。
The BrdUrd‐Hoechst method was used to analyze the interaction of various oxygen concentrations with BrdUrd substituted DNA with respect to cellular proliferation. At oxygen concentrations above 5%, human diploid fibroblast‐like cells and amniotic fluid fibroblast‐like cells showed reduced proliferation rates, which resulted from an increase in noncycling cells and from a permanent arrest of cells in the G2 phase of the cell cycle. At 35% oxygen the increase in noncyling cell fraction and the permanent arrest in G2 was strongly dependent upon the concentration of BrdUrd. Incorporation of BrdUrd into DNA, therefore, amplifies the adverse effects of increasing oxygen concentrations upon cell proliferation. The mechanism of this amplification might involve a free radical attack on DNA similar to the radiation sensitizing effect of BrdUrd.