Comparative analysis of the Arabidopsis pollen transcriptome
Comparative analysis of the Arabidopsis pollen transcriptome
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DOI：
10.1104/pp.103.020925
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发表时间：
2003-06-01
期刊：
影响因子：
7.4
通讯作者：
Twell, D
中科院分区：
文献类型：
--
作者：
Honys, D;Twell, D
我们提出了一个基因组范围内的观点，雄性配子体转录组在拟南芥基因芯片分析的基础上。与整个发育过程中孢子体的转录组相比，花粉转录组显示出降低的复杂性和独特的组成。我们鉴定了992种花粉表达的mRNA，其中近40%在花粉中特异性检测到。花粉转录组的功能组成的分析揭示了过量的表达的mRNA编码的蛋白质参与细胞壁代谢，细胞骨架，和信号和表达不足的mRNA参与转录和蛋白质合成。对于几个基因家族，我们观察到一个共同的模式，互斥的基因表达花粉和孢子体组织之间的不同基因家族成员。我们的研究结果提供了一个50倍的知识在拟南芥花粉中表达的基因。此外，我们还详细介绍了广泛的重叠（61%）的花粉转录组与孢子体，这提供了足够的潜力，通过配子体选择影响孢子体健身。
We present a genome-wide view of the male gametophytic transcriptome in Arabidopsis based on microarray analysis. In comparison with the transcriptome of the sporophyte throughout development, the pollen transcriptome showed reduced complexity and a unique composition. We identified 992 pollen-expressed mRNAs, nearly 40% of which were detected specifically in pollen. Analysis of the functional composition of the pollen transcriptome revealed the over-representation of mRNAs encoding proteins involved in cell wall metabolism, cytoskeleton, and signaling and under-representation of mRNAs involved in transcription and protein synthesis. For several gene families, we observed a common pattern of mutually exclusive gene expression between pollen and sporophytic tissues for different gene family members. Our results provide a 50-fold increase in the knowledge of genes expressed in Arabidopsis pollen. Moreover, we also detail the extensive overlap (61%) of the pollen transcriptome with that of the sporophyte, which provides ample potential to influence sporophytic fitness through gametophytic selection.