Genomic characterization and infectivity of a novel SARS-like coronavirus in Chinese bats.
Genomic characterization and infectivity of a novel SARS-like coronavirus in Chinese bats.
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DOI：
10.1038/s41426-018-0155-5
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发表时间：
2018-09-12
影响因子：
13.2
通讯作者：
Wang C
中科院分区：
文献类型：
--
作者：
Hu D;Zhu C;Ai L;He T;Wang Y;Ye F;Yang L;Ding C;Zhu X;Lv R;Zhu J;Hassan B;Feng Y;Tan W;Wang C
SARS冠状病毒（SARS-CoV）是2003年大规模SARS爆发的病原体，起源于蝙蝠。在蝙蝠中发现了许多类似 SARS 的冠状病毒（SL-CoV），特别是生活在中国、欧洲和非洲的蝙蝠。为了进一步了解SARS-CoV与其宿主之间的进化关系，2015年至2017年间从中国浙江省舟山市采集了334只蝙蝠。对保守的冠状病毒蛋白RdRp进行PCR扩增，在该地区26.65%的蝙蝠中检测到了冠状病毒，这一数字受到季节变化的影响。对舟山两种新型SL-CoV（ZXC21和ZC45）的全基因组分析显示，它们的基因组长度分别为29,732个核苷酸（nt）和29,802个nt，有13个开放阅读框（ORF）。这些结果显示，与人类/果子狸 SARS 冠状病毒有 81% 的核苷酸同一性，这比之前在中国观察到的蝙蝠 SL-CoV 的核苷酸同一性更远。重要的是，通过病原学测试，我们发现该病毒可以在乳鼠体内繁殖并引起疾病，进一步的研究表明，通过电子显微镜可以在乳鼠的大脑中观察到病毒样颗粒。因此，本研究增加了我们对蝙蝠携带的SL-CoV遗传多样性的认识，也为以乳鼠为动物模型研究SL-CoV跨物种传播的可能性提供了新的视角。
SARS coronavirus (SARS-CoV), the causative agent of the large SARS outbreak in 2003, originated in bats. Many SARS-like coronaviruses (SL-CoVs) have been detected in bats, particularly those that reside in China, Europe, and Africa. To further understand the evolutionary relationship between SARS-CoV and its reservoirs, 334 bats were collected from Zhoushan city, Zhejiang province, China, between 2015 and 2017. PCR amplification of the conserved coronaviral protein RdRp detected coronaviruses in 26.65% of bats belonging to this region, and this number was influenced by seasonal changes. Full genomic analyses of the two new SL-CoVs from Zhoushan (ZXC21 and ZC45) showed that their genomes were 29,732 nucleotides (nt) and 29,802 nt in length, respectively, with 13 open reading frames (ORFs). These results revealed 81% shared nucleotide identity with human/civet SARS CoVs, which was more distant than that observed previously for bat SL-CoVs in China. Importantly, using pathogenic tests, we found that the virus can reproduce and cause disease in suckling rats, and further studies showed that the virus-like particles can be observed in the brains of suckling rats by electron microscopy. Thus, this study increased our understanding of the genetic diversity of the SL-CoVs carried by bats and also provided a new perspective to study the possibility of cross-species transmission of SL-CoVs using suckling rats as an animal model.
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DOI：
10.1038/nrmicro2147
发表时间：
2009-06
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
11.4
作者：
Li, WH;Zhang, CS;Sui, JH;Kuhn, JH;Moore, MJ;Luo, SW;Wong, SK;Huang, IC;Xu, KM;Vasilieva, N;Murakami, A;He, YQ;Marasco, WA;Guan, Y;Choe, HY;Farzan, M
通讯作者：
Farzan, M
影响因子：
11.8
作者：
Cui J;Han N;Streicker D;Li G;Tang X;Shi Z;Hu Z;Zhao G;Fontanet A;Guan Y;Wang L;Jones G;Field HE;Daszak P;Zhang S
通讯作者：
Zhang S
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
9.4
作者：
Fouchier, RAM;Bestebroer, TM;Osterhaus, ADME
通讯作者：
Osterhaus, ADME