A tunable refractive index matching medium for live imaging cells, tissues and model organisms
A tunable refractive index matching medium for live imaging cells, tissues and model organisms
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DOI：
10.7554/elife.27240
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发表时间：
2017-07-14
期刊：
影响因子：
7.7
通讯作者：
Rink, Jochen C.
中科院分区：
文献类型：
--
作者：
Boothe, Tobias;Hilbert, Lennart;Rink, Jochen C.
在光学显微镜中，介质和样品之间的折射率不匹配会导致球差，这往往会限制穿透深度和分辨率。光学清除技术可以缓解这些失配，但到目前为止，它们仅限于固定样品。我们将碘黄醇作为一种无毒的介质补充剂，允许在活体样本中进行折射率匹配，从而显著改善活体成像的原代细胞培养物、浮游生物、斑马鱼和人脑器官的图像质量。
In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus substantially improves image quality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral organoids.