The glycosomal ATP-dependent phosphofructokinase of Trypanosoma brucei must have evolved from an ancestral pyrophosphate-dependent enzyme.
The glycosomal ATP-dependent phosphofructokinase of Trypanosoma brucei must have evolved from an ancestral pyrophosphate-dependent enzyme.
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布氏锥虫的糖体 ATP 依赖性磷酸果糖激酶必定是从祖先的焦磷酸依赖性酶进化而来。
DOI：
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
D. J. Rigden
中科院分区：
文献类型：
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作者：
P. Michels;N. Chevalier;F. Opperdoes;Mark H. Rider;D. J. Rigden
布氏锥虫含有ATP依赖性磷酸果糖激酶（PFK），位于其糖体中，糖体是过氧化物酶体样细胞器，隔离其大部分糖酵解酶。在本文中，我们报告的单拷贝基因编码这种酶的克隆和测序。其氨基酸序列与焦磷酸盐（PPi）依赖性PFKs比其他ATP依赖性PFKs更相似。系统发育分析表明，该酶必须已经从一个PPi依赖的祖先PFK，这改变了其磷酸供体特异性在进化过程中。该酶不再能够使用PPi作为磷酸底物，也不能像PPi-PFK通常那样催化逆反应。此外，细胞中存在高焦磷酸酶活性使得PPi不太可能在当今锥虫中充当自由能源。仍有待确定哪些突变是导致锥虫PFK磷酸化底物特异性改变的原因。由于其独特的进化历史，T.与其他ATP依赖性PFK相比，布氏PFK显示出许多结构差异，甚至在活性位点。这些差异为基于结构的杀锥虫药物设计提供了巨大的潜力。
Trypanosoma brucei contains an ATP-dependent phosphofructokinase (PFK), located in its glycosomes, which are peroxisome-like organelles sequestering the majority of its glycolytic enzymes. In this paper, we report the cloning and sequencing of the single-copy gene encoding this enzyme. Its amino-acid sequence is more similar to pyrophosphate (PPi)-dependent PFKs than to other ATP-dependent PFKs. A phylogenetic analysis suggests that the enzyme must have been derived from a PPi-dependent ancestral PFK, which changed its phospho-donor specificity during evolution. The enzyme is no longer capable of using PPi as phospho substrate, nor can it catalyze the reverse reaction as PPi-PFKs generally can. Moreover, the presence of a high pyrophosphatase activity in the cell renders it unlikely that PPi can function as free-energy source in present-day trypanosomes. It remains to be determined which mutations were responsible for the change in phospho-substrate specificity of the trypanosomatid PFK. As a result of its particular evolutionary history, the T. brucei PFK shows many structural differences, even at the active site, when compared with other ATP-dependent PFKs. These differences offer great potential for the structure-based design of trypanocidal drugs.
费氏丙酸杆菌焦磷酸依赖性磷酸果糖激酶参与底物结合和催化的碱性残基的鉴定。
DOI：
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发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Xu,J;Green,PC;Kemp,RG
通讯作者：
Kemp,RG
将萤火虫荧光素酶体内导入布氏锥虫糖体并进行 C 端靶向信号的突变分析。
影响因子：
3.3
作者：
Sommer,JM;Cheng,QL;Keller,GA;Wang,CC
通讯作者：
Wang,CC
嗜热脂肪芽孢杆菌磷酸果糖激酶基因的核苷酸序列以及与同源大肠杆菌基因的比较。
影响因子：
3.5
作者：
French,BA;Chang,SH
通讯作者：
Chang,SH