Dilute sulfuric acid pretreatment of transgenic switchgrass for sugar production.
Dilute sulfuric acid pretreatment of transgenic switchgrass for sugar production.
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DOI：
10.1016/j.biortech.2011.11.051
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发表时间：
2012
影响因子：
11.4
通讯作者：
Xu Zhou;Jiele Xu;Ziyu Wang;Jay J. Cheng;Ru-yu Li;R. Qu
中科院分区：
文献类型：
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作者：
Xu Zhou;Jiele Xu;Ziyu Wang;Jay J. Cheng;Ru-yu Li;R. Qu
传统的阿拉莫柳枝稷和它的转基因对应物减少/改性木质素进行稀硫酸预处理，以提高糖的生产。在150°C下，研究了酸浓度（0.75%、1%、1.25%）和停留时间（5、10、20、30 min）对柳枝稷预处理和酶解产糖的影响，并根据总糖得率和糖降解产物的生成量确定了各基因型柳枝稷的最佳预处理条件。结果表明，基因工程，虽然没有引起明显的木质素减少，导致在酸溶性木质素：酸不溶性木质素的比例大幅增加，这导致在预处理和酶解糖产量显着增加。在5-羟基呋喃甲醛和糠醛的联合阈值浓度（2.0g/L）升高时，常规柳枝稷和转基因柳枝稷10/9-40和11/5-47的总碳水化合物转化率分别达到75.9%、82.6%和82.2%。
Conventional Alamo switchgrass and its transgenic counterparts with reduced/modified lignin were subjected to dilute sulfuric acid pretreatment for improved sugar production. At 150°C, the effects of acid concentration (0.75%, 1%, 1.25%) and residence time (5, 10, 20, 30min) on sugar productions in pretreatment and enzymatic hydrolysis were investigated, with the optimal pretreatment conditions determined for each switchgrass genotype based on total sugar yield and the amounts of sugar degradation products generated during the pretreatment. The results show that genetic engineering, although did not cause an appreciable lignin reduction, resulted in a substantial increase in the ratio of acid soluble lignin:acid insoluble lignin, which led to considerably increased sugar productions in both pretreatment and enzymatic hydrolysis. At an elevated threshold concentration of combined 5-hydroxyfuranmethal and furfural (2.0g/L), the overall carbohydrate conversions of conventional switchgrass and its transgenic counterparts, 10/9-40 and 11/5-47, reached 75.9%, 82.6%, and 82.2%, respectively.