Dopamine-induced recruitment of dopamine D1 receptors to the plasma membrane
Dopamine-induced recruitment of dopamine D1 receptors to the plasma membrane
复制标题
DOI：
10.1073/pnas.95.10.5573
复制
发表时间：
1998-05-12
影响因子：
11.1
通讯作者：
Aperia, A
中科院分区：
文献类型：
--
作者：
Brismar, H;Asghar, M;Aperia, A
一般认为G蛋白偶联受体从细胞质向质膜的募集是一个组成性过程。我们在这里显示，通过使用共聚焦显微镜和亚细胞分级分离，至少有一个这样的受体，这种招聘是受管制的，而不是组成。将来自近端小管样细胞系LLCPK1细胞的细胞与D1激动剂、多巴胺前体或多巴胺代谢抑制剂一起孵育，以增加细胞中多巴胺的可用性。这三个程序中的每一个都导致多巴胺D1受体从胞质溶胶快速易位到质膜。
The recruitment of G protein-coupled receptors from the cytoplasm to the plasma membrane generally is believed to be a constitutive process. We show here by the use of both confocal microscopy and subcellular fractionation that, for at least one such receptor, this recruitment is regulated and not constitutive. Cells from a proximal tubular-like cell line, LLCPK1 cells, were incubated with either a D1 agonist, a dopamine precursor, or an inhibitor of dopamine metabolism to increase dopamine availability in the cell. Each of the three procedures led to a rapid translocation of dopamine D1 receptors from the cytosol to the plasma membrane.