Quantitation over time of tomato yellow leaf curl geminivirus DNA in its whitefly vector
Quantitation over time of tomato yellow leaf curl geminivirus DNA in its whitefly vector
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DOI：
10.1094/phyto.1997.87.6.610
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发表时间：
1997-06-01
期刊：
影响因子：
3.2
通讯作者：
Bosco, D
中科院分区：
文献类型：
--
作者：
Caciagli, P;Bosco, D
用地高辛标记的特异性DNA探针，通过荧光定量斑点杂交法研究了番茄黄化曲叶双生病毒（TYLCV）DNA在烟粉虱（Bemisia tabaci）中的积累量。大组的女性粉虱被允许喂养4，12，24，或48小时的TYLCV感染的番茄植物，然后转移到TYLCV免疫黄瓜植物。在采集期间和采集后的不同时间对昆虫进行取样，并检测TYLCV-DNA含量。TYLCV-DNA测定在整个昆虫上或分别在头部加前胸（包括唾液腺）和腹部上进行。TYLCV DNA的最大量，平均从0.5至1.6纳克每只昆虫，总是在收购期结束时达到。然后，平均量每天减少约1 - 2%，在采集期结束后20天内仍可明显检测到。只有一些在腹部呈TYLCV阳性的粉虱在头部加前胸呈阳性。在身体的两个部位，TYLCV DNA在采集期结束后的18天内仍然可以检测到，这表明TYLCV DNA在昆虫组织中的保留时间比感染性所指示的时间长得多。
The amount of tomato yellow leaf curl geminivirus (TYLCV) DNA that accumulated in the vector Bemisia tabaci was studied by quantitative chemiluminescent dot-blot assay, using digoxigenin-labeled specific DNA probes. Large groups of female whiteflies were allowed to feed for 4, 12, 24, or 48 h on TYLCV-infected tomato plants and then were transferred to TYLCV-immune cucumber plants. Insects were sampled at different times during and after acquisition access and tested for TYLCV-DNA content. TYLCV-DNA assays were done either on whole insects or on the head plus prothorax (to include salivary glands) and abdomen separately. The maximum amount of TYLCV DNA, averaging from 0.5 to 1.6 ng per insect, was always attained at the end of the acquisition period. The mean amount then decreased by about 1 to 2% per day, remaining clearly detectable up to 20 days after the end of the acquisition period. Only some whiteflies that were TYLCV-positive in the abdomen were positive for head plus prothorax. In both parts of the body, TYLCV DNA remained detectable up to 18 days after the end of the acquisition period, showing that TYLCV DNA remains in insect tissues much longer than infectivity indicates.