A third set of genes regulates thymic selection.
A third set of genes regulates thymic selection.
复制标题
第三组基因调节胸腺选择。
DOI：
10.1101/sqb.1989.054.01.017
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Kanagawa,O
中科院分区：
文献类型：
--
作者：
Palmer,E;Woodland,DL;Happ,MP;Bill,J;Kanagawa,O
方法：所有小鼠购自杰克逊实验室（缅因州）或在国家犹太中心（科罗拉多）的设施中繁殖。先前已描述了本研究中使用的T细胞杂交体（Bill et al. 1988）。它们由B10、B10的促分裂原（伴刀豆球蛋白-A）刺激的外周T细胞产生。Q和B10。BR小鼠与BW 5147融合，并如前所述保持在培养物中（Kappler等人，1981）。选择用于本研究的是24个表达Va 5 mRNA的杂交体。对来自T细胞杂交瘤2 HB 51的β-链eDNA进行测序显示，该杂交体表达符合读框的V，5.1 mRNA转录物。2HB51 8是选择用于高水平Va 5 RNA的2 HB 51的亚克隆。
METHODSMice. All mice were purchased from Jackson Laboratories (Maine) or bred in the facilities of National Jewish Center (Colorado).T-ceU hybrids. The T-cell hybrids used in this study have been described previously (Bill et al. 1988). They were generated from mitogen (concanavalin-A)-stimulated peripheral T cells from B10, B10. Q, and B10. BR mice that were fused to BW5147 and maintained in culture as described previously (Kappler et al. 1981). Selected for use in this study were 24 hybrids that expressed mRNA for Va5. Sequencing the/3-chain eDNA from the T-cell hybridoma 2HB51 showed that this hybrid expresses an in-frame V, 5.1 mRNA transcript. 2HB51. 8 is a subclone of 2HB51 selected for high levels of Va5 RNA.