Transcription Factor Sp1 Is Essential for the Regulation of the Porcine Caveolin-1 Gene
Transcription Factor Sp1 Is Essential for the Regulation of the Porcine Caveolin-1 Gene
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转录因子 Sp1 对于猪 Caveolin-1 基因的调节至关重要
DOI：
10.1089/dna.2010.1202
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发表时间：
2011-07-01
影响因子：
3.1
通讯作者：
Mo, Delin
中科院分区：
文献类型：
--
作者：
Chen, Weiquan;Chen, Yaosheng;Mo, Delin
Caveolin-1（CAV-1）是调节胆固醇的Caveolae的关键结构组分。由于缺乏对CAV-1基因调控的认识，利用转基因技术调控猪肉胆固醇含量的研究受到了阻碍。为了研究猪CAV-1的调控机制，从猪基因组DNA中分离了包含CAV-1 5 '侧翼区的DNA片段，并进行了序列测定。荧光素酶报告基因检测结果表明，CAV-1基因在-213 ~-20位核苷酸的区域内存在5个顺式作用元件，其中-123 ~-114为核心启动子。电泳迁移率变动分析表明，潜在的Sp1蛋白结合到这个核心启动子。本研究旨在系统阐明猪CAV-1的转录调控机制，为进一步研究CAV-1与胆固醇的相互作用奠定基础。
Caveolin-1 (CAV-1) is a key structural component of caveolae that regulates cholesterol. Employing transgenic techniques to regulate the cholesterol content of pork through CAV-1 is hindered by our lack of knowledge about its regulation. To investigate the regulatory mechanism of porcine CAV-1, a DNA segment containing the 5'-flanking region of CAV-1 was isolated from porcine genomic DNA and sequenced. The luciferase reporter assay detected five cis-acting elements for efficient expression of the CAV-1 gene at the region spanning nucleotides -213 to -20 with serially deleted 50-flanking sequences and site-directed mutants, -123 to -114 was the core promoter. The electrophoretic mobility shift assay demonstrated potential binding of Sp1 protein to this core promoter. The purpose of this study is to systematically elucidate the transcriptional regulation mechanism of porcine CAV-1 and to contribute to the investigation of the interaction between CAV-1 and cholesterol.