Purification and characterization of a cofactor that controls the oxidative phase of the pentose phosphate cycle in liver and other tissues of rat.
Purification and characterization of a cofactor that controls the oxidative phase of the pentose phosphate cycle in liver and other tissues of rat.
复制标题
控制大鼠肝脏和其他组织中磷酸戊糖循环氧化阶段的辅因子的纯化和表征。
DOI：
10.1016/s0304-4165(89)80012-1
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
M. Freire
中科院分区：
文献类型：
--
作者：
G. García;M. Nogueira;M. Freire
We have recently reported the presence, in rat liver, of a cofactor characterized as a protein ofMr105, which cooperates with GSSG to prevent the inhibition of glucose-6-phosphate dehydrogenase by NADPH [6]. The inhibition that this coenzyme also exerts on 6-phosphogluconate dehydrogenase is similarly prevented by a cofactor-GSSG system. The activity of the cofactor increases in the livers of rats fed on carbohydrate-rich diets. Purification of the components in rat liver homogenate by ion-exchange chromatography and preparative Polyacrylamide gel electrophoresis showed that the deinhibitory effect on both dehydrogenases is exerted by the same cofactor. The purified cofactor appeared as a unique protein ofMr37 · 103in SDS-polyacrylamide gel electrophoresis. Rat kidney and adipose tissue were the only nonhepatic tissues showing a cofactor-GSSG deinhibitory effect on both dehydrogenases of the oxidative phase of the pentose phosphate cycle. The deinhibitory activity, also corresponding with a cellular component ofMr105, was only diet-inducible in adipose tissue. The neutralization of the kidney and adipose tissue deinhibitory activity by rat liver cofactor antibodies suggested that there was a structural relationship between the cofactors prepared from these tissues.
NADP 结合蛋白导致人红细胞中 NADP 的可用性和乙状释放减少。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kirkman,HN;Gaetani,GF;Clemons,EH
通讯作者：
Clemons,EH