Alteration of T4 lysozyme structure by second-site reversion of deleterious mutations.
Alteration of T4 lysozyme structure by second-site reversion of deleterious mutations.
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通过有害突变的第二位点回复改变 T4 溶菌酶结构。
DOI：
10.1002/pro.5560061115
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Hardy,LW
中科院分区：
文献类型：
--
作者：
Poteete,AR;Rennell,D;Bouvier,SE;Hardy,LW
分离并表征了抑制通过单个氨基酸取代引入T4溶菌酶的缺陷的突变。在调查的53个主要位点中，8个产生了第二位点回复突变体;共获得了18种不同的突变体。大多数的恢复性突变发挥全球性的影响，一般增加溶菌酶的功能，在一些主要的突变体的情况。其中六个是更具体的，只抑制某些特定的有害的初级取代，或减少功能的溶菌酶轴承，否则无害的初级取代。推测在Asp 20和Ala 98位置具有主要取代的蛋白质的一些变体具有显著改变的结构。
Mutations that suppress the defects introduced into T4 lysozyme by single amino acid substitutions were isolated and characterized. Among 53 primary sites surveyed, 8 yielded second‐site revertants; a total of 18 different mutants were obtained. Most of the restorative mutations exerted global effects, generally increasing lysozyme function in a number of primary mutant contexts. Six of them were more specific, suppressing only certain specific deleterious primary substitutions, or diminishing the function of lysozymes bearing otherwise nondeleterious primary substitutions. Some variants of proteins bearing primary substitutions at the positions of Asp 20 and Ala 98 are inferred to have significantly altered structures.