Controlled Tubular Unit Formation from Collagen Film for Modular Tissue Engineering.
Controlled Tubular Unit Formation from Collagen Film for Modular Tissue Engineering.
复制标题
用于模块化组织工程的胶原膜受控管状单元形成。
DOI：
10.1021/acsbiomaterials.6b00468
复制
发表时间：
2017
影响因子：
5.8
通讯作者：
Fu,Jianping
中科院分区：
文献类型：
--
作者：
Sang,Jianming;Li,Xiang;Shao,Yue;Li,Zida;Fu,Jianping
自底向上或模块化组织工程是在体外制备仿生结构的新兴方法之一，包括在将其组装成功能组织结构之前，将小组织单元作为构建块进行制造。在这里，我们报道了一种微尺度的组织工程方法，通过细胞收缩力诱导细胞负载胶原膜以可控的方式自折叠来产生管状组织单位。胶原膜的自折叠是由于哺乳动物细胞固有的收缩特性引起的。我们详细探讨了胶原凝胶浓度、细胞密度和固有的细胞收缩力对满载细胞的胶原膜的自折叠和管状结构形成的独立影响。通过实验和理论建模，我们进一步证明了在胶原膜背面集成脊阵结构在引入结构各向异性从而控制胶原膜自折叠方向方面的有效性。我们使用脊阵列结构引入机械各向异性，从而促进管状组织单元形成的方法可以扩展到其他生物材料系统，从而为模块化组织工程应用提供了一种简单而有效的制备管状组织单元的方法。
Bottom-up or modular tissue engineering is one of the emerging approaches to prepare biomimetic constructsin vitro, involving fabrication of small tissue units as building blocks before assembling them into functional tissue constructs. Herein, we reported a microscale tissue engineering approach to generate tubular tissue units through cellular contractile force induced self-folding of cell-laden collagen films in a controllable manner. Self-folding of cell-laden collagen films was driven by film contraction resulted from intrinsic contractile property of adherent mammalian cells seeded in collagen films. We explored in detail independent effects of collagen gel concentration, cell density, and intrinsic cellular contractility on self-folding and tubular structure formation of cell-laden collagen films. Through both experiments and theoretical modeling, we further demonstrated the effectiveness of integrating ridge array structures onto the backside of collagen films in introducing structural anisotropy and thus controlling self-folding directions of collagen films. Our approach of using ridge array structures to introduce mechanical anisotropy and thus promote tubular tissue unit formation can be extended to other biomaterial systems and thus provide a simple yet effective way to prepare tubular tissue units for modular tissue engineering applications.