Functional gene cloning and characterization of the SsmE multidrug efflux pump from Serratia marcescens
Functional gene cloning and characterization of the SsmE multidrug efflux pump from Serratia marcescens
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DOI：
10.1248/bpb.31.516
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发表时间：
2008-03-01
影响因子：
2
通讯作者：
Tsuchiya, Tomofusa
中科院分区：
文献类型：
--
作者：
Minato, Yusuke;Shahcheraghi, Fereshteh;Tsuchiya, Tomofusa
我们从粘质沙雷氏菌的染色体DNA中克隆了一个多药耐药基因，并测定了其核苷酸序列。我们将该基因命名为ssmE。SsmE的氨基酸序列与小的多药耐药（SMR）型多药外排泵具有高度的相似性。大肠杆菌KAM 32，一种药物超敏感突变体，用携带ssmE基因的质粒pESM 437转化的细胞显示出结构无关的抗菌剂的最低抑菌浓度升高。E. coli KAM 32/pESM 437中的Etidium的能量依赖性外排增加。逆转录聚合酶链反应（RT-PCR）分析表明，ssmE在S. marcescens。
We cloned a gene responsible for multidrug resistance from chromosomal DNA of Serratia marcescens, and determined the nucleotide sequence. We designated the gene as ssmE. The deduced amino acid sequence of SsmE showed high similarity with the small multidrug resistance (SMR)-type multidrug efflux pumps. Cells of Escherichia coli KAM32, a drug hyper-susceptible mutant, transformed with a plasmid pESM437 carrying the ssmE gene showed elevated minimum inhibitory concentrations of structurally unrelated antimicrobial agents. E. coli KAM32/pESM437 showed elevated energy dependent efflux: of ethidium. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis revealed that ssmE was expressed in cells of S. marcescens.