Introduction of Laser Interference Lithography to Make Nanopatterned Surfaces for Fundamental Studies on Stem Cell Response.
Introduction of Laser Interference Lithography to Make Nanopatterned Surfaces for Fundamental Studies on Stem Cell Response.
复制标题
引入激光干涉光刻来制作纳米图案表面，用于干细胞反应的基础研究
DOI：
10.1021/acsbiomaterials.8b00060
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发表时间：
2018
影响因子：
5.8
通讯作者：
Groth T.
中科院分区：
文献类型：
--
作者：
Ekambaram B.K;Niepel M.S;Fuhrmann B;Schmidt G;Groth T.
细胞外基质（ECM）是一种纳米结构的环境，为各种细胞功能提供化学、机械和地形刺激。本文采用激光干涉光刻技术（LIL）在硅上生成三种不同尺寸的六边形排列金纳米结构，研究特征尺寸对人脂肪源性干细胞（hADSC）粘附、生长和分化的影响。自组装单层（SAM）被用来用长链聚乙二醇（PEG）钝化背景硅表面，而金纳米结构被巯基癸酸（MUDA）激活，只会直接吸附蛋白质和细胞粘附结构。结果表明，纳米结构的大小和距离影响hADSC的扩散，随着特征尺寸的增加，细胞大小减小，这也与局灶黏附的表达和小GTPase RhoA的存在相对应。这些早期事件的影响，与外向内信号转导有关，通过在较小的特征尺寸上增强细胞生长和对细胞分化的明显影响可见。由于化学和地形线索的精确控制，该系统为研究材料地形对干细胞行为的影响提供了巨大的潜力，这可能为定制植入物和组织工程支架表面的应用铺平道路。
The extracellular matrix (ECM) is a nanostructured environment that provides chemical, mechanical, and topographical stimuli for various cellular functions. Here, we introduce the application of laser interference lithography (LIL) to generate hexagonally arranged gold nanostructures of three different dimensions on silicon to study the effect of feature dimensions on human adipose-derived stem cells (hADSC) in terms of adhesion, growth, and differentiation. Self-assembled monolayers (SAM) were used to passivate the background silicon surface with a long-chain polyethylene glycol (PEG), whereas the gold nanostructures were activated with mercaptoundecanoic acid (MUDA) to direct protein adsorption and cell adhesive structures to them, only. It was possible to show that the size and distance of the nanostructures affected the spreading of hADSC with a decrease of cell size with the increase of feature dimensions, which corresponded also to the expression of focal adhesions and presence of the small GTPase RhoA. Effects of these early events, related to outside-in signal transduction, were visible by an enhanced cell growth on smaller feature dimensions and distinct effects on cell differentiation. Because of the precise control of chemical and topographical cues, the presented system offers great potential to study effects of material topography on stem cell behavior, which may pave the way for applications in tailoring surfaces of implants and tissue engineering scaffolds.