Nickel accumulation by immobilized biofilm of Cirobacter sp. containing cell-bound polycrystalline hydrogen uranyl phosphate
Nickel accumulation by immobilized biofilm of Cirobacter sp. containing cell-bound polycrystalline hydrogen uranyl phosphate
复制标题
Cirobacter sp. 固定化生物膜的镍积累。
作者：
G. Bašnáková;J. Finlay;L. Macaskie
磷酸氢铀（HUO_2 PO_4：HUP），酶促沉积在柠檬酸杆菌N14细胞上作为生物膜固定在陶瓷拉西环上，在流通柱中，通过插层离子交换从稀水溶液中以磷酸铀酰镍的形式定量去除镍。通过用柠檬酸盐缓冲液或用含有UO 22和磷酸盐供体（甘油2-磷酸盐）的缓冲液洗涤来再生负载镍的柱，这得到额外的结晶HUP存款，用于随后改善镍去除。在Ni的选择性解吸过程中没有发生铀释放，证明了柱内生物膜的完整性。使用陶瓷载体来制造人工的、生物无机的离子交换剂是新颖的，并且使用固体基质克服了限制凝胶固定化细胞用于大规模过程的应用的机械稳定性问题。© Rapid Science有限公司1998
Hydrogen uranyl phosphate (HUO2PO4: HUP), deposited enzymatically on Citrobacter N14 cells immobilized as biofilm on ceramic Raschig rings in a flow-through column, removed nickel quantitatively from dilute aqueous solution in the form of nickel uranyl phosphate, via intercalative ion exchange. Nickel-loaded columns were regenerated by washing either with citrate buffer or with buffer containing UO22 and phosphate donor (glycerol 2-phosphate), this giving additional crystalline HUP deposit for subsequent improvement of nickel removal. No uranium release occurred during selective desorption of Ni, proving the integrity of the biofilm within the column. The use of ceramic supports to manufacture an artificial, bioinorganic, ion exchanger is novel and the use of solid matrices overcomes the problems of mechanical stability which limit the applications of gel-immobilized cells for large-scale processes. © Rapid Science Ltd. 1998