Norcantharidin Induces HL-60 Cells Apoptosis In Vitro.
Norcantharidin Induces HL-60 Cells Apoptosis In Vitro.
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DOI：
10.1155/2012/154271
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Chen JX
中科院分区：
文献类型：
--
作者：
Jiang YM;Meng ZZ;Yue GX;Chen JX
去甲斑蝥素（NCTD）是斑蝥素的去甲基化形式，斑蝥素是mylabris的活性物质，已知具有抗癌潜力。本实验旨在探讨NCTD对HL-60细胞的诱导凋亡作用。方法。流式细胞仪检测NCTD对体外培养HL-60细胞的细胞毒性、细胞周期和凋亡的影响。结果。NCTD处理48h后，HL-60细胞的生长受到明显抑制。24 h后出现细胞凋亡高峰。5、10、50 μmol/L NCTD作用24 h后，G1期细胞比例先下降后上升，S期和G2 + M期细胞比例均被阻断。结论。NCTD能诱导HL-60细胞凋亡，抑制细胞分裂，且多米诺骨牌效应与给药时间和剂量明显相关。
Norcantharidin (NCTD) is the demethylated form of cantharidin, which is the active substance of mylabris, and is known to have anticancer potentials. The aim of this paper was to assess the apoptosis-inducing effect of NCTD on HL-60 cells. Methods. The effects of NCTD were detected by flow cytometer on the cell toxicity, cell cycle, and apoptosis of HL-60 cells cultured in vitro. Results. After 48-hour treatment with NCTD, the growth of HL-60 cells was inhibited significantly. The summit of apoptosis appeared after 24 hours. The percentage of the cells in G1 phase decreased and then increased in S and G2 + M phase, while the S and G2 + M phases were blocked after treatment with 5, 10, and 50 μmol/L NCTD for 24 hours. Conclusions. NCTD can induce the apoptosis of HL-60 cells and inhibit the fissiparism, and the domino effect was obviously correlated with the time and dosage.
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