NUCLEOTIDE-SEQUENCE OF MOUSE SATELLITE DNA
NUCLEOTIDE-SEQUENCE OF MOUSE SATELLITE DNA
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DOI：
10.1093/nar/9.3.683
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发表时间：
1981-01-01
影响因子：
14.9
通讯作者：
ALTENBURGER, W
中科院分区：
文献类型：
--
作者：
HORZ, W;ALTENBURGER, W
通过分析与卫星DMA重复单元相对应的Sau96l限制性内切片段，确定了未克隆小鼠卫星DNA的核苷酸序列。得到了一个明确的234bp序列。Sau96l限制性酶切中二聚体卫星片段的前250个碱基序列几乎完全对应于两个串联排列的单体序列，其中包括位于中心的完整的Sau96l位点。这与一种假设是一致的，即在非克隆DNA的序列分析中无法检测到的低水平的分歧是导致二聚体片段出现的原因。对TaqI易感的5%的Sau96单体的大部分序列也已确定，并发现与原型序列完全一致。单体序列是内部重复的，由八个分散的亚重复组成。这种分化模式对小鼠卫星DNA的进化理论具有有趣的意义。
The nudeotide sequence of uncloned mouse satellite DNA has been determined by analyzing Sau96l restriction fragments that correspond to the repeat unit of the satellite DMA. An unambiguous sequence of 234 bp has been obtained. The sequence of the first 250 bases from dimeric satellite fragments present in Sau96l limit digests corresponds almost exactly to two tandemly arranged monomer sequences including a complete Sau96l site inthe center. This is in agreement with the hypothesis that a low level of divergence which cannot be detected in sequence analyses of uncloned DNA is responsible for the appearance of dimeric fragments. Most of the sequence of the 5% fraction of Sau96 monomers that are susceptible to TaqI has also been determined and has been found to agree completely with the prototype sequence. The monomer sequence is internally repetitious being composed of eight diverged subrepeats. The divergence pattern has interesting implications for theories on the evolution of mouse satellite DNA.