DISTRIBUTION AND DIFFUSION OF SOLUTES IN ARTICULAR CARTILAGE
DISTRIBUTION AND DIFFUSION OF SOLUTES IN ARTICULAR CARTILAGE
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DOI：
10.1016/s0006-3495(70)86307-x
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发表时间：
1970-01-01
影响因子：
3.4
通讯作者：
MAROUDAS, A
中科院分区：
文献类型：
--
作者：
MAROUDAS, A
本文对关节软骨与外界溶液之间溶质的分布及其在软骨中的扩散系数进行了实验研究。溶质分为小离子、不带电荷的小分子和从葡萄糖到血红蛋白大小递增的不带电荷的分子。当软骨在生理盐水中平衡时，钠离子和氯离子的分布服从唐南平衡。然而，在浸在稀溶液中的软骨中，氯离子的浓度高于预测。这可能是由于软骨中存在一些微观区域，其中粘多糖耗尽，唐南排除不起作用。不带电荷的小分子如尿素的摩尔分配系数接近于1，这表明软骨中的所有水似乎都表现为溶剂水。对于较大的分子，分布以及扩散系数随着分子量的增加而减小，并且对固定电荷密度的变化非常敏感。结果已根据“空间排阻”原理进行解释。能够渗透到软骨中的最大分子是血红蛋白分子的大小。
An experimental study was made on the distribution of solutes between articular cartilage and external solution, and on their diffusivity in cartilage. The solutes were classed as small ions, small uncharged molecules, and uncharged molecules of increasing size ranging from glucose to hemoglobin. The distribution of sodium and chloride ions obeys the Donnan equilibrium when cartilage is equilibrated in physiological saline solution. However, in cartilage immersed in dilute solution the concentration of chloride ions is higher than predicted. This is probably due to the presence in cartilage of some microscopic regions depleted of mucopolysaccharide in which the Donnan exclusion does not operate. The molal distribution coefficients of small uncharged molecules like urea are close to unity, which indicates that all water in cartilage seems to behave as solvent water. For larger molecules the distribution as well as the diffusion coefficients decrease with increase in molecular weight and are very sensitive to variations in fixed charge density. The results have been interpreted on the basis of the "steric exclusion" principle. The largest molecules which can penetrate into cartilage are of the size of the hemoglobin molecule.