Relationship of superoxide dismutase to rotator cuff injury/tear in a rat model
Relationship of superoxide dismutase to rotator cuff injury/tear in a rat model
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DOI：
10.1002/jor.25141
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发表时间：
2021-07-11
影响因子：
2.8
通讯作者：
Ishijima, Muneaki
中科院分区：
文献类型：
--
作者：
Uehara, Hirohisa;Itoigawa, Yoshiaki;Ishijima, Muneaki
肩袖退变是导致肩袖撕裂的几个因素之一。氧化应激和超氧化物歧化酶已被报道与肩袖退变有关;然而，确切的机制仍不清楚。在这项研究中，我们研究了氧化应激和超氧化物歧化酶与肩袖退变的大鼠模型。采用84只大鼠建立胶原酶诱导的肩袖损伤模型（损伤模型）和肩袖撕裂模型（撕裂模型）。对照组分别给予生理盐水和仅三角肌切口。我们使用退化评分、二氢乙锭荧光强度（检测氧化应激）、基因表达和超氧化物歧化酶活性评价肩袖的退化形态。损伤和撕裂模型中的肩袖显著增加变性评分和二氢乙锭荧光强度。另一方面，超氧化物歧化酶亚型，超氧化物歧化酶1，超氧化物歧化酶活性的基因表达显着降低损伤模型，但在泪液模型中没有显着差异。这些结果表明，超氧化物歧化酶可能与撕裂后肩袖退变无关，但可能与无撕裂的肩袖退变有关。然而，我们发现肩袖退变涉及氧化应激，无论有无撕裂。基于这些发现，可以推测，根据肩袖退变的状态，不同的治疗方法可能是合适的，因为退变的机制可能不同。
Rotator cuff degeneration is one of the several factors that lead to rotator cuff tears. Oxidative stress and superoxide dismutase have been reported to be related to rotator cuff degeneration; however, the precise mechanism still remains unclear. In this study, we investigated the relationship of oxidative stress and superoxide dismutase to the degeneration of the rotator cuff using rat models. Eighty-four rats were used to create a collagenase-induced rotator cuff injury model (injury model) and a rotator cuff tear model (tear model). The controls were administered saline and had only a deltoid incision, respectively. We evaluated degeneration morphology of the rotator cuff using a degeneration score; dihydroethidium fluorescence intensity, which detects oxidative stress; gene expression; and superoxide dismutase activity. The rotator cuffs in the injury and tear models significantly increased degeneration scores and dihydroethidium fluorescence intensity. On the other hand, gene expression of superoxide dismutase isoform, superoxide dismutase 1, and superoxide dismutase activity were significantly decreased in the injury model but showed no significant difference in the tear model. These findings suggested that superoxide dismutase might not be associated with rotator cuff degeneration after tear but may be involved in degenerative rotator cuff without tear. However, we found that rotator cuff degeneration involves oxidative stress both with and without tear. Based on these findings, it is presumed that different treatments may be appropriate, depending on the state of rotator cuff degeneration, because the mechanisms of the degeneration may be different.