Chromatin immunoprecipitation in fission yeast.
Chromatin immunoprecipitation in fission yeast.
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DOI：
10.1007/978-1-61779-046-1_2
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发表时间：
2011
影响因子：
--
通讯作者：
Thomas A. Volpe;Jessica Demaio
中科院分区：
文献类型：
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作者：
Thomas A. Volpe;Jessica Demaio
在过去的10年里，出现了大量关于异染色质的性质和调控的信息。这一快速进步在很大程度上要归功于染色质免疫沉淀或“芯片”等技术的发展，这种技术可以分析染色质的结构。进一步的技术进步，如微阵列分析和最近的深度测序技术，使芯片成为一种更强大的工具。芯片使研究人员能够识别蛋白质相互作用和/或特定基因组位置上各种染色质修饰的存在。
A tremendous amount of information regarding the nature and regulation of heterochromatin has emerged in the past 10 years. This rapid progress is largely due to the development of techniques such as chromatin immunoprecipitation or “ChIP,” which allow analysis of chromatin structure. Further technological advances such as microarray analysis and, more recently, deep sequencing technologies, have made ChIP an even more powerful tool. ChIP allows the investigator to identify protein interactions and/or the presence of various chromatin modifications at specific genomic loci.