Cell delivery in regenerative medicine: The cell sheet engineering approach
Cell delivery in regenerative medicine: The cell sheet engineering approach
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DOI：
10.1016/j.jconrel.2006.06.022
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发表时间：
2006-11-28
影响因子：
10.8
通讯作者：
Okano, Teruo
中科院分区：
文献类型：
--
作者：
Yang, Joseph;Yamato, Masayuki;Okano, Teruo
最近，基于细胞的疗法已经发展成为再生医学的基础。迄今为止，用于细胞递送的一般方法涉及使用将单细胞悬浮液直接注射到靶组织中。此外，将细胞接种到生物可降解支架中的一般范例的组织工程也已经发展为用于重建各种组织和器官的方法。随着临床试验的成功，使用这些方法的再生疗法因此引起了极大的兴趣和关注。作为一种新的替代方法，我们已经开发了使用温度响应培养皿的细胞片工程，其允许培养细胞作为完整的片沿着其沉积的细胞外基质的非侵入性收获。使用这种方法，细胞片可以直接移植到宿主组织，而不使用支架或载体材料，或用于通过单个细胞片的分层来创建体外组织构建体。除了简单的移植之外，细胞片工程化的构建体也已应用于替代疗法，例如内窥镜移植、组合组织重建和多外科手术，以克服使用常规方法的再生疗法和细胞递送的局限性。(c)2006 Elsevier B.V.保留所有权利。
Recently, cell-based therapies have developed as a foundation for regenerative medicine. General approaches for cell delivery have thus far involved the use of direct injection of single cell suspensions into the target tissues. Additionally, tissue engineering with the general paradigm of seeding cells into biodegradable scaffolds has also evolved as a method for the reconstruction of various tissues and organs. With success in clinical trials, regenerative therapies using these approaches have therefore garnered significant interest and attention. As a novel alternative, we have developed cell sheet engineering using temperature-responsive culture dishes, which allows for the non-invasive harvest of cultured cells as intact sheets along with their deposited extracellular matrix. Using this approach, cell sheets can be directly transplanted to host tissues without the use of scaffolding or carrier materials, or used to create in vitro tissue constructs via the layering of individual cell sheets. In addition to simple transplantation, cell sheet engineered constructs have also been applied for alternative therapies such as endoscopic transplantation, combinatorial tissue reconstruction, and polysurgery to overcome limitations of regenerative therapies and cell delivery using conventional approaches. (c) 2006 Elsevier B.V. All rights reserved.