PHYCOBILISOME STRUCTURE IN THE CYANOBACTERIA MASTIGOCLADUS-LAMINOSUS AND ANABAENA SP PCC-7120
PHYCOBILISOME STRUCTURE IN THE CYANOBACTERIA MASTIGOCLADUS-LAMINOSUS AND ANABAENA SP PCC-7120
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DOI：
10.1111/j.1432-1033.1992.tb16857.x
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发表时间：
1992-05-01
期刊：
影响因子：
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通讯作者：
ZUBER, H
中科院分区：
文献类型：
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作者：
GLAUSER, M;BRYANT, DA;ZUBER, H
蓝藻Mastigocladus laminosus和Anabaena sp. PCC 7120的藻胆体与典型的三环藻、半环藻藻胆体有三个方面的不同。首先，通过SDS/PAGE比较了不同蓝藻的核-膜连接藻胆蛋白（L（CM））的大小，发现M的L（CM）的表观分子量为120 kDa。laminosus和鱼腥藻PCC 7120。这一观察结果表明，这些物种的多肽具有四个接头重复结构域。其次，M.在一些实施方案中，层状体显示含有至少三种，但最可能是四种不同的杆-核接头多肽（L（RC））。这些L（RC），其将外周杆连接到核心，从而使藻蓝蛋白/别藻蓝蛋白接触，已被确定和其特征在于通过N-末端氨基酸序列分析。此外，还对从M. laminosus和Anabaena sp. PCC 7120显示了与Calothrix sp. PCC 7601不同的类似结构，具有典型的六个外围杆。基于蛋白质分析结果和对[Isono，T. & Katoh，T.（1987）Arch. Biochem. Biophys. 256，317-324]，我们讨论了最近的研究结果对已建立的M. laminosus和鱼腥藻PCC 7120。多达八个周边杆建议辐射从一个修改的核心子结构，其中包含两个额外的周边别藻蓝蛋白六聚体等效物，作为核心近端光盘的两个周边杆。
Phycobilisomes of the cyanobacteria Mastigocladus laminosus and Anabaena sp. PCC7120 differ from typical tricylindrical, hemidiscoidal phycobilisomes in three respects. Firstly, size comparisons of the core-membrane linker phycobiliproteins (L(CM)) in different cyanobacteria by SDS/PAGE reveal an apparent molecular mass of 120 kDa for the L(CM) of M. laminosus and Anabaena sp. PCC7120. This observation suggests that the polypeptides of these species have four linker-repeat domains. Secondly, phycobilisomes of M. laminosus are shown to contain at least three, but most probably four, different rod-core linker polypeptides (L(RC)). These L(RC), which attach the peripheral rods to the core and thereby make phycocyanin/allophycocyanin contacts, have been identified and characterized by N-terminal amino acid sequence analysis. Additionally, electron microscopy of phycobilisomes isolated from M. laminosus and Anabaena sp. PCC7120 reveals similar structures which differ from those of Calothrix sp. PCC7601 with their typical six, peripheral rods. Based upon protein-analytical results and a reinterpretation of the data of [Isono, T. & Katoh, T. (1987) Arch. Biochem. Biophys. 256, 317-324], we discuss structural implications of recent findings on the established hemidiscoidal model for the phycobilisomes of M. laminosus and Anabaena sp. PCC7120. Up to eight peripheral rods are suggested to radiate from a modified core substructure which contains two additional peripheral allophycocyanin hexamer equivalents that serve as the core-proximal discs for two peripheral rods.